Region of neurone isolation/recording | Agonist | Antagonist/inhibitor | Receptor | Effect | Desensitization | Reference |
---|---|---|---|---|---|---|
Cerebellum | 2MeSATP > ADP > ATP > ADO ≫ α,β-meATP > AMP > UTP | GDPβS | P2Y | K+channel | — | Ikeuchi and Nishizaki, 1996a |
Inferior colliculus | 2MeSATP > ADP > ATP > AMP > α,β-meATP | GDPβS | P2Y | K+channel | — | Ikeuchi and Nishizaki, 1995b |
Superior colliculus | 2MeSATP > ADP > ADO > ATP ≫ AMP (UTP, α,β-meATP inactive) | — | P2Y | K+channel | — | Ikeuchi et al., 1995b |
Dorsal motor nucleus of vagus | ATP (α,β-meATP inactive) | Suramin, RB2 GDPβS-insensitive | P2X | Rapid inward current | No | Nabekura et al., 1995 |
Hippocampus | ATP | Suramin | — | Slow inward currents12-a | No | Inoue et al., 1992 |
Hippocampus | 2MeSATP > ATP > ADP > α,β-meATP | Suramin | — | Inward currents | — | Balachandran and Bennett, 1996 |
Hippocampus | ADP ≥ 2MeSATP > ATP > ADO ≫ AMP (UTP, α,β-meATP inactive) | GDPβS | P2Y | K+ channel, ↑ [Ca2+]i | — | Ikeuchiet al., 1996a,b |
Hippocampus | ATP = ADP ≫ AMP > α,β-meATP | ↑[Ca2+]i via PKC | P2Y | ↑ [Ca2+]i | — | Mironov, 1994 |
Hypoglossal nucleus | ATP | Suramin, PPADS | P2 | Excitation of hypoglossal nerve | — | Funk et al., 1997 |
ATP inactive | — | — | No inward currents | — | Nabekura et al., 1995 | |
Hypothalamus | ATP, ATPγS > α,β-meATP (ADP inactive) | Suramin | P2X | Rapid ↑ [Ca2+]i | Slow (>100 s) | Chen et al., 1994a |
Locus ceruleus | ATP, α,β-meATP | — | P2X | ↑ Firing, depolarization | No | Harms et al., 1992 |
Locus ceruleus | α,β-meATP, 2MeSATP | Suramin, α,β-meATP | P2X | ↑ Firing, depolarization | No | Tschöpl et al., 1992 |
Locus ceruleus | 2MeSATP > ATP = ADP > α,β-meATP | — | — | ↑ Conductance; depolarization | Slow | Shen and North, 1993 |
Locus ceruleus | α,β-meATP | Suramin, PPADS | P2X | Rapid depolarization | No | Nieber et al., 1997 |
Suramin, PPADS | P2X | Block of synaptic potentials | — | |||
Medial habenula | ATP, α,β-meATP | Suramin | P2X | Inward currents | Yes | Edwardset al., 1992 |
Suramin, α,β-meATP | P2X | Block of synaptic potentials | (>100 ms) | |||
Medial vestibular nucleus | α,β-meATP | Suramin, PPADS | P2X | ↑ Firing | No | Chessell et al., 1997 |
ADPβS | PPADS (not suramin) | P2Y | ↑ Firing | Slow | ||
Medulla | 2MeSATP > ADP > ATP ≥ | GDPβS | P2Y | K+channel | — | Ikeuchi et al., 1995a |
α,β-meATP ≥ AMP > UTP | ||||||
Mesencephalic nucleus | ATP inactive | — | — | Ineffective at evoking inward currents | — | Shen and North, 1993 |
Mesencephalic nucleus | ATP, ATPγS, α,β-meATP | Suramin | P2X | Inward currents | Slow | Khakhet al., 1997 |
Nucleus tractus solitarius | ATP | — | — | Inward currents | No | Ueno et al., 1992; Nabekura et al., 1995 |
Parabrachial nucleus | ATP inactive | — | — | No inward currents | — | Shen and North, 1993 |
Rostral ventrolateral | ATP, α,β-meATP | Suramin | P2X | ↑ Firing | No | Sun et al., 1992 |
medulla | No, Yes12-b | Ralevic et al., 1996 | ||||
Striatum | 2MeSATP > ATP ≥ α,β-meATP | Suramin, RB2, PTX | P2Y | Dopamine release | — | Zhang et al., 1995 |
Striatum | ATP ≫ 2MeSATP ≥ ADP > ADO > AMP | — | — | K+channel | — | Ikeuchi and Nishizaki, 1995a |
Substantia nigra | ATP | — | — | Inward currents at 1 in 12 neurones | — | Nabekuraet al., 1995 |
Supraoptic vasopressin neurones | ATP = α,β-meATP | Suramin | P2X | ↑ Firing | No12-c | Dayet al., 1993 |
— | Suramin | P2 | Block of excitation to vagus nerve stimulation | |||
Supraoptic magnocellular neurosecretory cells (hypothalamic) | α,β-meATP > ATP > UTP > 2MeSATP > βγ-meATP | PPADS | P2X | Depolarization, ↑ input conductance | No | Hiruma and Bourque, 1995 |
Tuberomammillary nucleus | ATP ≥ 2MeSATP ≫ α,β-meATP ≥ ADP | — | P2X | Rapid inward cation current | No | Furukawa et al., 1994 |
Dorsal horn of spinal | ATP | — | P2X | Inward currents | Yes | Jahr and Jessel, 1983 |
cord | ATP | — | — | ↑ Excitability | — | Fyffe and Perl, 1984 |
ATP, ATPγS | Suramin | P2X | Rapid inward currents | Partial | Li and Perl, 1995 | |
Spinal cord neurones | 2MeSATP ≥ ATP ≥ ADP ≫ ADP (AMP, UTP, α,β-meATP inactive) | GDPβS | P2Y | K+currents | — | Ikeuchi and Nishizaki, 1996b |
↵12-a A rapid inward current is also observed, but is blocked by a non-NMDA receptor antagonist.
↵12-b Desensitization observed in a subpopulation of α,β-meATP-sensitive neurones.
↵12-c Reproducible responses to ATP on rapid application at <1 min intervals.
GDPβS, guanosine-5′-O-(2-thiodiphosphate); G protein inhibitor; PTX, pertussis toxin; RB2, reactive blue 2.