Table 6

Shown are examples of liver-specific genes that contain a regulatory element with a HNF-6 binding site. The species of the investigated gene with its regulatory sequence as well as the respective references are indicated. The positions of the HNF-6 binding sites have preferably been taken from published DNase I footprinting studies, if available. The next preference is for chemical modifications and the last for gel retardation assays. In case of different positional information for both DNA strands, the more upstream position has been taken for the 5′-border and the more downstream position for the 3′-border of the site. If not stated otherwise, the position numbers generally refer to the transcription start site (t.s.s.). Occasionally they may refer to the translation start codon stated as ATG or to a defined restriction site. When the authors emphasized a specific motif within the published regulatory sequence, it is written in capitals whereas the rest of the sequence is written in lowercase letters. → indicates a continuing sequence in the next line.

HNF-6 Binding Sites
GeneRegulatory ElementGene RegionPosition of Binding SiteFirst PositionSpeciesReference
α1-AntitrypsinTCCATTGATTTAG5′-Region−195 to −184t.s.s. M. musculus Samadani and Costa, 1996
α-FetoproteincaAAGTCAATAAag5′-Region−6103 to −6090t.s.s. R. norvegicus Lannoy et al., 1998; Samadani et al., 1996
CYP2C12gcaaaATATTGATTTttatggtgPromoter−53 to −30t.s.s. R. norvegicus Lannoy et al., 1998
CYP2C13AATATTGATTCTG−53 to −41t.s.s. R. norvegicus Samadani and Costa, 1996
Phosphoenolpyruvate carboxykinasecaaagttTAGTCAATCAaacgttg5′-Region−263 to −240t.s.s. R. norvegicus Lannoy et al., 1998; Jacquemin et al., 1999
6-Phosphofructo-2-kinase/fructose-2,6-bisphosphataseGctttgAAATCAATTTcaaagPromoter, site IV−216 to −196t.s.s. R. norvegicus Lannoy et al., 1998; Jacquemin et al., 1999; Lemaigre et al., 1996
agcttcaaacaaacaaaaaAAATCCATAActttcaIntron 1, GRU (glucocorticoid response unit)
Tryptophan oxygenaseTCTATTGATTTAT5′-Region−220 to −208t.s.s. R. norvegicus Samadani and Costa, 1996
TTR (transthyretin, prealbumin)CTAAGTCAATAAT5′-Region, HNF3S element−111 to −82t.s.s. M. musculus Samadani and Costa, 1996; Costa and Grayson, 1991