Table 6

Cannabinoid-induced inhibition of central and peripheral neurotransmitter release

TransmitterTissue Preparation or Brain AreaTransmitter-Releasing StimulusReferences
In vivo
ACh6-a Rat medial-prefrontal cortexNone Gessa et al., 1998a
Rat hippocampusNone6-b
GABA6-c Rat striatumNone Tersigni and Rosenberg, 1996
In vitro
AChRat hippocampal slicesES6-d
Rat hippocampal & frontal cortical synaptosomesK+ or Ca2+ Gifford et al., 2000
Mouse hippocampal or cerebrocortical slicesES or Ca2+ 6-e
Guinea pig intestinal tissue (MPLM)ES6-f
NAGuinea pig cerebrocortical slicesES Schlicker et al., 1997
Human and guinea pig hippocampal slicesES or Ca2+ Schlicker et al., 1997
Guinea pig hippocampal slicesNMDA or kainate Kathmann et al., 1999a
Guinea pig hypothalamic slicesES Schlicker et al., 1997
Guinea pig cerebellar slicesES Schlicker et al., 1997
Guinea pig retinal discsES or Ca2+ Schlicker et al., 1996
Guinea pig bronchial slicesES Vizi et al., 2001
Human atrial appendage segmentsES Molderings et al., 1999
Rat atriaES Ishac et al., 1996
Rat heartES Kurihara et al., 2001
Rat vas deferensES Ishac et al., 1996
Mouse vas deferensES Trendelenburg et al., 2000
Mouse cultured sympathetic neuronsES Göbel et al., 2000
DARat striatal slicesNMDA Kathmann et al., 1999a
Rat striatal slicesES Cadogan et al., 1997
Guinea pig retinal discsES Schlicker et al., 1996
5-HTMouse cerebrocortical slicesES or Ca2+ Nakazi et al., 2000
Mouse hypothalamic slicesES Kathmann et al., 1999b
GABAHuman hippocampal slicesES Katona et al., 2000
Rat hippocampal slicesES Katona et al., 1999
GABA6-c Rat or mouse hippocampal slicesES6-g
Slices of rat or mouse amygdala (BLC)ES Katona et al., 2001
1y cultures of neonatal rat hippocampal cellsNone Irving et al., 2000
1y cultures of neonatal rat hippocampal cellsES Ohno-Shosaku et al., 2001
Rat striatal slicesES Szabo et al., 1998
Rat midbrain slices (SNR)ES6-h
Rat brain slices (RVM)ES Vaughan et al., 1999
Rat cerebellar slicesNone Takahashi and Linden, 2000
Rat cerebellar slicesES Kreitzer and Regehr, 2001b
Rat brain slices (PAG)ES Vaughan et al., 2000
Rat brain slices (shell region of NAc)ES Hoffman and Lupica, 2001
Mouse brain slices (NAc)ES Manzoni and Bockaert, 2001
Rat spinal trigeminal nucleus pars caudalis (SG)ES Jennings et al., 2001
Guinea pig intestinal tissue (MPLM)Ethylenediamine Begg et al., 2002
Glu6-c Rat prefrontal cortical slicesES Auclair et al., 2000
Rat brain slices (PAG)ES Vaughan et al., 2000
Mouse brain slices (NAc)ES Robbe et al., 2001
1y cultures of rat hippocampal cellsLow [Mg2+]o 6-i
1y cultures of rat hippocampal cellsES Sullivan, 1999
Mouse hippocampal slices6-j ES6-k
Rat or mouse cerebellar slicesES6-l
Rat striatal slicesES6-m
Rat midbrain slices (SNR)ES Szabo et al., 2000
Rat spinal cord slices (SG)ES Morisset and Urban, 2001
1y cultures of rat cerebellar granule cellsLow [Mg2+]o Irving et al., 2001
Gly6-c Rat spinal trigeminal nucleus pars caudalis (SG)ES Jennings et al., 2001
d-Asp1y cultures of rat cerebellar granule cellsK+ Breivogel et al., 1999
CCKRat hippocampal slicesK+ Beinfeld and Connolly, 2001
  • ES, electrical stimulation; [Mg2+]o, extracellular magnesium concentration; MPLM, myenteric plexus-longitudinal muscle preparation; BLC, basolateral complex; NAc, nucleus accumbens; PAG, periaqueductal gray; RVM, rostral ventromedial medulla; SG, substantia gelatinosa; SNR, substantia nigra pars reticulata; ACh, acetylcholine; DA, dopamine; d-Asp,d-aspartate; NA, noradrenaline; 1y, primary.

  • 6-a  ACh collected by microdialysis.

  • 6-bGessa et al., 1997, 1998a; Carta et al., 1998; Nava et al., 2000, 2001.

  • 6-c  Indirect electrophysiological evidence for decreased transmitter release: in some of these investigations, there was also evidence that cannabinoids inhibited spontaneous as well as evoked release of GABA or Glu.

  • 6-dGifford and Ashby, 1996; Gifford et al., 1997a,b; 1999; Kathmann et al., 2001a.

  • 6-eNakazi et al., 2000; Kathmann et al., 2001a,b.

  • 6-fPertwee et al., 1996; Coutts and Pertwee, 1997; Mang et al., 2001.

  • 6-gHájos et al., 2000, 2001;Hoffman and Lupica, 2000; Wilson and Nicoll, 2001.

  • 6-hChan and Yung, 1998; Chan et al., 1998.

  • 6-iShen et al., 1996; Shen and Thayer, 1998a,b; 1999.

  • 6-j  Signs ofR-(+)-WIN55212-induced inhibition of glutamate release have been observed in tissue from both wild-type and CB1 −/− mice.

  • 6-kMisner and Sullivan, 1999;Hájos et al., 2001.

  • 6-lLévénès et al., 1998; Kreitzer and Regehr, 2001a; Maejima et al., 2001.

  • 6-mGerdeman and Lovinger, 2001; Huang et al., 2001.