TABLE 13

KV3.2 channels

Channel name KV3.2
Description Voltage-gated potassium channel, delayed rectifier
Other names RKShIIIA,1 Raw1,2 Kv3.2a,3 rKv3.2b and rKv3.2c4
Molecular information Human: 613aa, NM_139136 (transcript variant 1), chr. 12q14.1,5 KCNC2, GeneID: 3747, PMID: 811111821
Mouse: AC121610 (genomic), chr. 10
Rat: 613aa, NM_139216 (transcript variant a), chr. 7q12–22
Associated subunits None
Functional assays Electrophysiology
Current Delayed rectifier
Conductance 16–20pS16
Ion selectivity K+
Activation Va = 13 mV; ka = 7–7.5 mV1; ton = 10–90% (40 mV) 4 ms; τoff 2.9 ms (–60 mV)16
Inactivation Very slow16
Activators None
Gating inhibitors None
Blockers Tetraethyammonium (0.1 mM),6 4-aminopyridine (0.1 mM),6 8-bromo-cGMP,7 3-isobutyl-1-methylxanthine,6 D-NONOate,7 verapamil (11 μM),8 ShK19
Radioligands None
Channel distribution Brain (fast-spiking GABAergic interneurons of the neocortex, hippocampus, and caudate; terminal fields of thalamocortical projections),9,10,11,12 islets,13 mesenteric artery, Schwann cells14
Physiological functions Probably in heteromeric complexes with KV3.1; important for the high-frequency firing of fast spiking GABAergic interneurons17 and GABA release via regulation of action potential duration in presynaptic terminals18; modulated by protein kinase A in vitro and in vivo10,20
Mutations and pathophysiology See “Comments”
Pharmacological significance Not established
Comments Fast deactivation; knockout mice show specific alterations in their cortical electroencephalographic patterns and an increased susceptibility to epileptic seizures consistent with an impairment of a cortical inhibitory mechanism15; mammalian Shaw-related family

  • aa, amino acids; chr., chromosome; D-NONOate, 1,1-diethyl-2-hydroxy-2-nitrosohydrazine; ShK, Stychodactyla helianthus toxin.

  • ↵1. McCormack T, Vega-Saenz de Miera EC, and Rudy B (1991) Molecular cloning of a member of a third class of Shaker-family K+ channel genes in mammals. Proc Natl Acad Sci USA 87:5227-5231

  • ↵2. Rettig J, Wunder F, Stocker M, Lichtinghagen R, Mastiaux F, Beckh S, Kues W, Pedarzani P, Schroter KH, Ruppersberg JP, et al. (1992) Characterization of a Shaw-related potassium channel family in rat brain. EMBO J 11:2473-2486

  • ↵3. Ponce A, Vega-Saenz de Miera E, Kentros C, Moreno H, Thornhill B, and Rudy B (1997) K+ channel subunit isoforms with divergent carboxy-terminal sequences carry distinct membrane targeting signals. J Membr Biol 159:149-159

  • ↵4. Luneau C, Wiedmann R, Smith JS, and Williams JB (1991) Shaw-like rat brain potassium channel cDNA's with divergent 3′ ends. FEBS Lett 288:163-167

  • ↵5. Haas M, Ward DC, Lee J, Roses AD, Clarke V, D'Eustachio P, Lau D, Vega-Saenz de Miera E, and Rudy B (1993) Localization of Shaw-related K+ channel genes on mouse and human chromosomes. Mamm Genome 4:711-715

  • ↵6. Lien CC, Martina M, Schultz JH, Ehmke H, and Jonas P (2002) Gating, modulation and subunit composition of voltage-gated K+ channels in dendritic inhibitory interneurones of rat hippocampus. J Physiol 538:405-419

  • ↵7. Moreno H, Vega-Saenz de Miera E, Nadal MS, Amarillo Y, and Rudy B (2001) Modulation of Kv3 potassium channels expressed in CHO cells by a nitric oxide-activated phosphatase. J Physiol 530:345-358

  • ↵8. Madeja M, Muller V, Musshoff U, and Speckmann EJ (2001) Sensitivity of native and cloned hippocampal delayed-rectifier potassium channels to verapamil. Neuropharmacology 39:202-210

  • ↵9. Weiser M, Vega-Saenz de Miera E, Kentros C, Moreno H, Franzen L, Hillman D, Baker H, and Rudy B (1994) Differential expression of Shaw-related K+ channels in the rat central nervous system. J Neurosci 14:949-972

  • ↵10. Moreno H, Kentros C, Bueno E, Weiser M, Hernandez A, Vega-Saenz de Miera E, Ponce A, Thornhill W, and Rudy B (1995) Thalamocortical projections have a K+ channel that is phosphorylated and modulated by cAMP-dependent protein kinase. J Neurosci 15:5486-5501

  • ↵11. Chow A, Erisir A, Farb C, Nadal MS, Ozaita A, Lau D, Welker E, and Rudy B (1999) K+ channel expression distinguishes subpopulations of parval-bumin- and somatostatin-containing neocortical interneurons. J Neurosci 19:9332-9345

  • ↵12. Tansey EP, Chow A, Rudy B, and McBain CJ (2002) Develo pMental expression of potassium-channel subunit Kv3.2 within subpopulations of mouse hippocampal inhibitory interneurons. Hippocampus 12:137-148

  • ↵13. Roe MW, Worley JF 3rd, Mittal AA, Kuznetsov A, Dasgupta S, Mertz RJ, Witherspoon SM 3rd, Blair N, Lancaster ME, McIntyre MS, et al. (1996) Expression and function of pancreatic β-cell delayed rectifier K+ channels: role in stimulus-secretion coupling. J Biol Chem 271:32241-32246

  • ↵14. Sobko A, Peretz A, Shirihai O, Etkin S, Cherepanova V, Dagan D, and Attali B (1998) Heteromultimeric delayed-rectifier K+ channels in Schwann cells: develo pMental expression and role in cell proliferation. J Neurosci 18:10398-10408

  • ↵15. Lau D, Vega-Saenz de Miera EC, Contreras D, Ozaita A, Harvey M, Chow A, Noebels JL, Paylor R, Morgan JI, Leonard CS, and Rudy B (2000) Impaired fast-spiking, suppressed cortical inhibition, and increased susceptibility to seizures in mice lacking Kv3.2 K+ channel proteins. J Neurosci 20:9071-9085

  • ↵16. Rudy B, Chow A, Lau D, Amarillo Y, Ozaita A, Saganich M, Moreno H, Nadal MS, Hernandez-Pineda R, Hernandez-Cruz A, et al. (1999) Contributions of Kv3 channels to neuronal excitability. Ann NY Acad Sci 868:304-343

  • ↵17. ErisirA, Lau D, Rudy B, and Leonard CS (1999) The function of specific K+ channels in sustained high frequency firing of fast-spiking neocortical interneurons. J Neurophysiol 82:2476-2489

  • ↵18. Goldberg EM, Watanabe S, Chang SY, Joho RH, Huang ZJ, Leonard CS, and Rudy B (2005) Specific functions of synaptically localized potassium channels in synaptic transmission at the neocortical GABAergic fast-spiking cell synapse. J Neurosci 25:5230-5235

  • ↵19. Yan L, Herrington J, Goldberg E, Dulski PM, Bugianesi RM, Slaughter RS, Banerjee P, Brochu RM, Priest BT, Kaczorowski GJ, et al. (2005) Stichodactyla helianthus peptide, a pharmacological tool for studying Kv3.2 channels. Mol Pharmacol 67:1513-1521

  • ↵20. Atzori M, Lau D, Tansey EP, Chow A, Ozaita A, Rudy B, and McBain CJ (2000) H2 histamine receptor-phosphorylation of Kv3.2 modulates interneuron fast spiking. Nat Neurosci 3:791-798

  • ↵21. Haas M, Ward DC, Lee J, Roses AD, Clarke V, D'Eustachio P, Lau D, Vega-Saenz de Miera E, and Rudy B (1993) Localization of Shaw-related K+ channel genes on mouse and human chromosomes. Mamm Genome 4:711-715