TRPV5 channel

Channel name TRPV51,2,3,4,5
Description Calcium-selective TRP channel
Other names ECaC1, CaT2
Molecular information Human unigene: Hs0.283369, chr. 7q35
Mouse unigene: Mm0.135734, chr. 6 B1
Associated subunits NHERF, S100 protein, TRPV6
Functional assays Patch-clamp, calcium imaging
Current Inwardly rectifying, calcium-sensitive cation channel
Conductance 75pS in calcium-free solution
Erev in saline solution >50 mV
Ion selectivity Ca2+ > Ba2+ > Sr2+ > Mn2+; PNa/PCa = 1:107
Activation Mechanism not established, constitutively active
Inactivation Not established
Activators None
Gating inhibitors None
Blockers Ruthenium red (100 nM), La3+
Radioligands None
Channel distribution Intestine, kidney, placenta
Physiological functions Not established
Mutations and pathophysiology D542 is crucial for calcium sensitivity
Pharmacological significance Not established
Comments One of only two known TRP channels (with TRPV6) that is relatively highly Ca2+-selective
  • chr., chromosome; NHERF, Na+/H+ exchange regulatory factor.

  • 1. Vennekens R, Hoenderop GJ, Prenen J, Stuiver M, Willems PHGM, Droogmans G, Nilius B, and Bindels RJM (2000) Permeation and gating properties of the novel epithelial Ca2+ channel. J Biol Chem 275:3963-3969

  • 2. Nilius B, Vennekens R, Prenen J, Hoenderop JG, Bindels RJ, and Droogmans G (2000) Whole-cell and single channel monovalent cation currents through the novel rabbit epithelial Ca2+ channel ECaC. J Physiol 527 (Pt 2):239-248

  • 3. Hoenderop JGJ, van der Kemp AWCM, Hartog A, van de Graaf SFJ, van Os CH, Willems PHGM, and Bindels RJM (1999) Molecular identification of the apical Ca2+ channel in 1,25-dihydroxyvitamin D3-responsive epithelia. J Biol Chem 274:8375-8378

  • 4. van de Graaf SF, Hoenderop JG, Gkika D, Lamers D, Prenen J, Rescher U, Gerke V, Staub O, Nilius B, and Bindels RJ (2003) Functional expression of the epithelial Ca2+ channels (TRPV5 and TRPV6) requires association of the S100A10-annexin 2 complex. EMBO J 22:1478-1487

  • 5. Embark HM, Setiawan I, Poppendieck S, van de Graaf SF, Boehmer C, Palmada M, Wieder T, Gerstberger R, Cohen P, Yun CC, et al. (2004) Regulation of the epithelial Ca2+ channel TRPV5 by the NHE regulating factor NHERF2 and the serum and glucocorticoid inducible kinase isoforms SGK1 and SGK3 expressed in Xenopus oocytes. Cell Physiol Biochem 14:203-212