TRPV5 channel
| Channel name | TRPV51,2,3,4,5 |
| Description | Calcium-selective TRP channel |
| Other names | ECaC1, CaT2 |
| Molecular information | Human unigene: Hs0.283369, chr. 7q35 |
| Mouse unigene: Mm0.135734, chr. 6 B1 | |
| Associated subunits | NHERF, S100 protein, TRPV6 |
| Functional assays | Patch-clamp, calcium imaging |
| Current | Inwardly rectifying, calcium-sensitive cation channel |
| Conductance | 75pS in calcium-free solution |
| Erev in saline solution >50 mV | |
| Ion selectivity | Ca2+ > Ba2+ > Sr2+ > Mn2+; PNa/PCa = 1:107 |
| Activation | Mechanism not established, constitutively active |
| Inactivation | Not established |
| Activators | None |
| Gating inhibitors | None |
| Blockers | Ruthenium red (100 nM), La3+ |
| Radioligands | None |
| Channel distribution | Intestine, kidney, placenta |
| Physiological functions | Not established |
| Mutations and pathophysiology | D542 is crucial for calcium sensitivity |
| Pharmacological significance | Not established |
| Comments | One of only two known TRP channels (with TRPV6) that is relatively highly Ca2+-selective |
chr., chromosome; NHERF, Na+/H+ exchange regulatory factor.
↵1. Vennekens R, Hoenderop GJ, Prenen J, Stuiver M, Willems PHGM, Droogmans G, Nilius B, and Bindels RJM (2000) Permeation and gating properties of the novel epithelial Ca2+ channel. J Biol Chem 275:3963-3969
↵2. Nilius B, Vennekens R, Prenen J, Hoenderop JG, Bindels RJ, and Droogmans G (2000) Whole-cell and single channel monovalent cation currents through the novel rabbit epithelial Ca2+ channel ECaC. J Physiol 527 (Pt 2):239-248
↵3. Hoenderop JGJ, van der Kemp AWCM, Hartog A, van de Graaf SFJ, van Os CH, Willems PHGM, and Bindels RJM (1999) Molecular identification of the apical Ca2+ channel in 1,25-dihydroxyvitamin D3-responsive epithelia. J Biol Chem 274:8375-8378
↵4. van de Graaf SF, Hoenderop JG, Gkika D, Lamers D, Prenen J, Rescher U, Gerke V, Staub O, Nilius B, and Bindels RJ (2003) Functional expression of the epithelial Ca2+ channels (TRPV5 and TRPV6) requires association of the S100A10-annexin 2 complex. EMBO J 22:1478-1487
↵5. Embark HM, Setiawan I, Poppendieck S, van de Graaf SF, Boehmer C, Palmada M, Wieder T, Gerstberger R, Cohen P, Yun CC, et al. (2004) Regulation of the epithelial Ca2+ channel TRPV5 by the NHE regulating factor NHERF2 and the serum and glucocorticoid inducible kinase isoforms SGK1 and SGK3 expressed in Xenopus oocytes. Cell Physiol Biochem 14:203-212