K2P10.1 channels

Channel name K2P10.1
Description Two-pore domain potassium channel subunit; open rectifier1
Other names KCNK10, TREK-2
Molecular information Human: 538aa (see `Comments'), NM_138317, chr0.14q31, KCNK10, GeneID: 54207, PMID: 107479111
Rat: 538aa, NM_023096, kcnk10
Mouse: not cloned
Associated subunits Not established
Functional assays Electrophysiological
Current Open rectifier2
Conductance 100pS (see “Comments”)
Ion selectivity Not established
Activation See “Comments”
Inactivation See “Comments”
Activators Arachidonic acid, docosahexaenoic acid, linoleic acid, lysophosphatidylcholine,3 intracellular acidification, volatile anesthetics: halothane (∼1 mM), isoflurane (∼1 mM); riluzole (∼1 mM), heat,6 mechanical stress3
Gating inhibitors None
Blockers Quinidine (100 mM), PKA, PKC
Radioligands None
Channel distribution Kidney, pancreas, prostate, thymus, liver, heart (see “Comments”)2
Physiological functions See “Comments”4,5
Mutations and pathophysiology Not established
Pharmacological significance Not established
Comments Activation and deactivation with voltage steps seem to be instantaneous; splice variants have been identified in human and rat; the rat variant is reported to have a conductance of 68pS and to be expressed in brain; K2P10-like currents are observed in cerebellar granular neurons, magnocellular neurosecretory cells of rat supraoptic nucleus,4,5 rat cortical astrocytes,7 and insulin-secreting MIN6 cells8
  • aa, amino acids; chr., chromosome; PKA, protein kinase A; protein kinase C.

  • 1. Bang H, Kim Y, and Kim D (2000) TREK-2, a new member of the mechano-sensitive tandem-pore K+ channel family. J Biol Chem 275:17412-17419

  • 2. Gu W, Schlichthorl G, Hirsch JR, Engels H, Karschin C, Karschin A, Derst C, Steinlein OK, and Daut J (2002) Expression pattern and functional characteristics of two novel splice variants of the two-pore-domain potassium channel TREK-2. J Physiol 539:657-668

  • 3. Lesage F, Terrenoire C, Romey G, and Lazdunski M (2000) Human TREK2, a 2P domain mechano-sensitive K+ channel with multiple regulations by polyunsaturated fatty acids, lysophospholipids, and Gs, Gi, and Gq protein-coupled receptors. J Biol Chem 275:28398-28405

  • 4. Han J, Truell J, Gnatenco C, and Kim D (2002) Characterization of four types of background potassium channels in rat cerebellar granule neurons. J Physiol 542:431-444

  • 5. Han J, Gnatenco C, Sladek CD, and Kim D (2003) Background and tandem-pore potassium channels in magnocellular neurosecretory cells of the rat supraoptic nucleus. J Physiol 546:625-639

  • 6. Kang DW, Choe CY, and Kim D (2005) Thermosensitivity of the two-pore domain K+ channels TREK-2 and TRAAK. J Physiol 564:103-116

  • 7. Gnatenco C, Han JH, Snyder AK, and Kim D (2002) Functional expression of TREK-2 K+ channel in cultured astrocytes. Brain Res 931:56-67

  • 8. Kang DW, Choe C, and Kim D (2004) Functional expression ofTREK-2 in insulin-secreting MIN6 cells. Biochem Biophys Res Commun 323:323-331