TRPC5 channel
| Channel name | TRPC51,2,3,4,5,6,7 |
| Description | TRP receptor-operated channel |
| Other names | TRP5, CCE2 |
| Molecular information | Human unigene: Hs0.247868, chr. Xq23 |
| Mouse unigene: Mm0.328378, chr. X | |
| Associated subunits | Forms heteromultimers with TRPC1 and TRPC4 |
| Functional assays | Patch-clamp, calcium imaging |
| Current | Nonselective, double rectification |
| Conductance | ∼38pS as homomer, ∼5pS as TRPC1/C5 heteromer |
| Ion selectivity | PNa/PCa = 1:9,1 1:1.82 |
| Activation | Stimulation of Gq-coupled receptors, receptor tyrosine kinases |
| Inactivation | Not established |
| Activators | Activation of Gq-coupled receptors is known to activate TRPC5 current through activation of PLCγ; La3+ (100 μM) augments current |
| Gating inhibitors | None |
| Blockers | mM La3+, 2-APB |
| Radioligands | None |
| Channel distribution | Brain, lung, placenta, testis |
| Physiological functions | Not established |
| Mutations and pathophysiology | Not established |
| Pharmacological significance | Not established |
| Comments | Channel properties of heteromeric TRPC1/TRPC4 or TRPC1/TRPC5 are distinct from those of TRPC4 or TPRC5 homomers; rapid insertion of vesicles in growth cones; regulator of neurite morphology |
chr., chromosome; RTK, receptor tyrosine kinase.
↵1. Okada T, Shimizu S, Wakamori M, Maeda A, Kurosaki T, Takada N, Imoto K, and Mori Y (1998) Molecular cloning and functional characterization of a novel receptor-activated TRP Ca2+ channel from mouse brain. J Biol Chem 273:10279-10287
↵2. Schaefer M, Plant TD, Obukhov AG, Hofmann T, Gudermann T, and Schultz G (2000) Receptor-mediated regulation of the nonselective cation channels TRPC4 and TRPC5. J Biol Chem 275:17517-17526
↵3. Strübing C, Krapivinsky G, Krapivinsky L, and Clapham DE (2001) TRPC1 and TRPC5 form a novel cation channel in mammalian brain. Neuron 29:645-655
↵4. Strübing C, Krapivinsky G, Krapivinsky L, and Clapham DE (2003) Formation of novel TRPC channels by complex subunit interactions in embryonic brain. J Biol Chem 278:39014-39019
↵5. Bezzerides VJ, Ramsey IS, Greka A, Kotecha SA, and Clapham DE (2004) Rapid vesicular translocation and insertion of TRP channels. Nat Cell Biol 6:709-720
↵6. Greka A, Navarro B, Oancea E, Duggan A, and Clapham DE (2003) TRPC5 is a regulator of hippocampal neurite length and growth cone morphology. Nat Neurosci 6:837-845
↵7. Lee YM, Kim BJ, Kim HJ, Yang DK, Zhu MH, Lee KP, So I, and Kim KW (2003) TRPC5 as a candidate for the nonselective cation channel activated by muscarinic stimulation in murine stomach. Am J Physiol Gastrointest Liver Physiol 284:G604-G616