TABLE 1

Actions of Aβ on nAChRs Direct actions of Aβ on nAChRs. The results of actions of Aβ or Aβ fragments on in situ or heterologously expressed nAChRs are summarized, along with a summary of how the Aβ was prepared (if given in the original report), and which concentrations were tested. Both activation and inhibition are reported by different laboratories, even for the same receptor subtype expressed in oocytes.

nAChR Species Expression System/Tissue Aβ Fragment Aβ Concentration Aβ Preparation Actions Reference
α3β2 Human X. laevis oocytes 1–40, 1–42 10 nM 5% Acetic acid No action Pym et al., 2005
α3β2 Human X. laevis oocytes 25–35 10 nM Glacial acetic acid Inhibits Pym et al., 2005
α2β2 X. laevis oocytes 1–42 Inhibits Lamb et al., 2005
α4α5β2 X. laevis oocytes 1–42 Inhibits Lamb et al., 2005
α4β2 Human SHEP cell line 1–42 1 nM Water Inhibits Wu et al., 2004
α4β2 X. laevis oocytes 1–42 Inhibits Lamb et al., 2005
α4β2 Human X. laevis oocytes 1–40, 1–42 10 nM 5% Acetic acid Potentiates Pym et al., 2005
α4β2 Human X. laevis oocytes 25–35 10 nM Glacial acetic acid Inhibits Pym et al., 2005
α7 Human Binding to transfected SK-N-MC cells 1–42 N.A. Not given Binds with high affinity Wang et al., 2000
α7 Rat Binding to transfected SH- SY5Y cells, and to rat brain 1–42 N.A. DMSO (unaggregated) No binding Small et al., 2007
α7 Rat X. laevis oocytes 1–42 1–100 pM HEPES pH8 Activates Dineley et al., 2002
α7 Human X. laevis oocytes 1–42 DMSO or acetic acid Inhibits Grassi et al., 2003
α7 Rat Presynaptic terminals isolated from rat hippocampus and neocortex 1–42, 12–28 1 pM Physiologic al saline Activates Dougherty et al., 2003
α7 Mouse Mouse prefrontal cortex 1–42, 12–28 1 pM–1 nM Not given Activates (indirect evidence: increases dopamine secretion) Wu et al., 2007
α7 Human X. laevis oocytes 1–42 10 nM 5% acetic acid Inhibits Pym et al., 2005
α7 Human X. laevis oocytes 25–35 10 nM Glacial acetic acid Inhibits Pym et al., 2005
α7 X. laevis oocytes 1–42 ? No inhibition Lamb et al., 2005
α7 muscle type Torpedo sp. X. laevis oocytes 1–42 100 nM Not given Inhibits Tozaki et al., 2002
α7-L248T Human X. laevis oocytes 1–42 DMSO or acetic acid Activates Grassi et al., 2003
α7-L250T X. laevis oocytes Activates Dineley et al., 2002
Non-α7 Rat Rat basal Forebrain neurones 25–35 4 μM Water Activates Fu and Jhamandas, 2003
Non-α7 Rat Rat basal Forebrain neurons 1–42 100 nM Water Activates Fu and Jhamandas, 2003
Single-channel Nicotinic Receptor currents Rat Rat Hippocampal interneurons 1–42 2 μM Not given Inhibits Pettit et al., 2001

  • DMSO, dimethyl sulfoxide; N.A., not applicable