Binding of [125I]BNtxA in transfected HEK cells
[125I]BNtxA binding was determined in HEK cells transfected with either the 6TM variant mMOR-1G, ORL1, or both. No binding was detectable in the cells transfected with either of the proteins alone. However, high-affinity binding was determined in cells cotransfected. From the Majumdar et al. (2011).
| Transfection | [125I]BNtxA Binding KD |
|---|---|
| nM | |
| mMOR-1G alone | No binding detected |
| ORL1 alone | No binding detected |
| mMOR-1G + ORL1 | 0.24 ± 0.03 |