TABLE 3

ASIC protein interaction partners

Interaction Partner	ASIC Subtypes Concerned	Site of interaction on ASIC (C or N Terminus)^{^a}	Consequences	System	References
A kinase-anchoring protein (AKAP) 79/150 and calcineurin	1a, 2a	ASIC2a: N & C, ASIC1a: C	AKAP 150 binding increases ASIC currents; calcineurin decreases ASIC currents.	Transfected cortical neurons or CHO cells	Chai et al., 2007
Channel-interacting PDZ domain protein (CIPP)	3	C, PDZ	Enhances peak current amplitude of ASIC3	Transfected COS cells	Anzai et al., 2002
PICK1	1a, 2a, 2b	C, PDZ	Affects localization of ASIC1a and allows ASIC2a current increase by PKC.	Mainly recombinant expression systems	Baron et al., 2002; Duggan et al., 2002; Hruska-Hageman et al., 2002; Deval et al., 2004
PICK1	1a, 2a, 2b	C, PDZ	Allows current increase of ASIC2b/3 channels by PICK1-dependent PKC phosphorylation of ASIC3.	Mainly recombinant expression systems
Lin7B	3	C, PDZ	Increases ASIC3 cell surface expression and current amplitude	Transfected CHO cells	Hruska-Hageman et al., 2004
MAGI-1b (membrane-associated guanylate kinase)	3	C, PDZ^{^b}	Increases ASIC3 current amplitude	Transfected CHO cells	Hruska-Hageman et al., 2004
PIST	3	C, PDZ^{^b}	Increases ASIC3 current amplitude	Transfected CHO cells	Hruska-Hageman et al., 2004
PSD95	3	C	Reduces ASIC3 cell surface expression and current amplitude	Transfected CHO cells	Hruska-Hageman et al., 2004; Eshcol et al., 2008; Zha et al., 2009
Stomatin	1, 2, 3	NR	Inhibits ASIC3 current amplitude and accelerates ASIC2a desensitization		Price et al., 2004
Stomatin-like proteins (STOML)	1, 2, 3	NR	ASIC- and STOML-subtype–dependent, inhibition of current amplitude and acceleration of desensitization	Transfected cells, neurons	Lapatsina et al., 2012; Kozlenkov et al., 2014
Na⁺/H⁺ exchanger regulatory factor (NHERF)	3	C, PDZ^{^b}	Increases ASIC current amplitude	Various cell lines	Deval et al., 2006
Annexin II light chain p11.1	1a	N	Increases cell surface expression and current amplitude	DRG neurons and transfected cells	Donier et al., 2005
α-Actinin	1a	C	Effect on ASIC1a current amplitude and properties, depending on actinin subtype	Cell lines and neurons	Schnizler et al., 2009
High-conductance Ca²⁺- and voltage-activated K⁺ (BK) channel	1a	NR	Reciprocal current inhibition	Transfected cells and neurons	Petroff et al., 2008, 2012
P2X channels	3	NR	ATP sensitizes ASIC3 for activation by protons	Neurons and transfected cells	Birdsong et al., 2010

NR, not resolved
↵^a “PDZ” in this column indicates that the interacting protein binds to the ASIC PDZ binding domain.
↵^b ASIC3 PDZ binding domain is different from that of ASIC1 and ASIC2. These proteins did not bind to ASIC1 or ASIC2.