TABLE 1

Crystal structures of the catalytic domains of mACs

PDB ID	C1a sequence	C2a sequence	Metal A Metal B^{^a}	Gα_s^b	ATP-site	FSK-site	Res.^c	Reference
1AB8^d	—	2C2 (rat)	—	—	—	FSK	2.20	Zhang et al., 1997
1AB8^d	—	871-1090	—	—	—	FSK	2.20	Zhang et al., 1997
1AZS	5C1 (dog)	2C2 (rat)	—	+	—	Methylpiperazino-FSK	2.30	Tesmer et al., 1997
1AZS	361-575	870-1081	—	GTPγS	—	Methylpiperazino-FSK	2.30	Tesmer et al., 1997
1CJK	5C1 (dog)	2C2 (rat)	Mg²⁺	+	Adenosine-5′-RP-(α-thio-PPP)	FSK	3.00	Tesmer et al., 1999
1CJK	364-580	870-1081	Mn²⁺	GTPγS	Adenosine-5′-RP-(α-thio-PPP)	FSK	3.00	Tesmer et al., 1999
1CJT	5C1 (dog)	2C2 (rat)	Mg²⁺	+	2’,3′-Dideoxy-ATP	FSK	2.80	Tesmer et al., 1999
1CJT	364-580	870-1081	Mn²⁺	GTPγS	2’,3′-Dideoxy-ATP	FSK	2.80	Tesmer et al., 1999
1CJU	5C1 (dog)	2C2 (rat)	Mg²⁺	+	2’,3′-Dideoxy-ATP	FSK	2.80	Tesmer et al., 1999
1CJU	364-580	870-1081	Mg²⁺	GTPγS	2’,3′-Dideoxy-ATP	FSK	2.80	Tesmer et al., 1999
1CJV	5C1 (dog)	2C2 (rat)	Zn²⁺	+	2’,3′-Dideoxy-ATP	FSK	3.00	Tesmer et al., 1999
1CJV	364-580	870-1081	Mg²⁺	GTPγS	2’,3′-Dideoxy-ATP	FSK	3.00	Tesmer et al., 1999
1CS4	5C1 (dog)	2C2 (rat)	—	+	2’-Deoxyadenosine-3′-P + PP	FSK	2.50	Tesmer et al., 2000
1CS4	361-580	870-1081	Mg²⁺	GTPγS	2’-Deoxyadenosine-3′-P + PP	FSK	2.50	Tesmer et al., 2000
1CUL	5C1 (dog)	2C2 (rat)	Mg²⁺	+	2’-Deoxyadenosine-3′-P	FSK	2.40	Tesmer et al., 2000
1CUL	364-580	874-1081	Mg²⁺	GTPγS	2’-Deoxyadenosine-3′-P	FSK	2.40	Tesmer et al., 2000
1TL7	5C1 (dog)	2C2 (rat)	Mn²⁺	+	3′-MANT-GTP	FSK	2.80	Mou et al., 2005
1TL7	364-580	870-1081	Mn²⁺	GTPγS	3′-MANT-GTP	FSK	2.80	Mou et al., 2005
1UOH	5C1 (dog)	2C2 (rat)	Mg²⁺	+	3′-MANT-GTP	FSK	2.90	Mou et al., 2005
1UOH	361-580	870-1081	Mg²⁺	GTPγS	3′-MANT-GTP	FSK	2.90	Mou et al., 2005
2GVD	5C1 (dog)	2C2 (rat)	Mn²⁺	+	TNP-ATP	Methylpiperazino-FSK	2.90	Mou et al., 2006
2GVD	363-580	870-1081	Mn²⁺	GTPγS	TNP-ATP	Methylpiperazino-FSK	2.90	Mou et al., 2006
2GVZ	5C1 (dog)	2C2 (rat)	Mn²⁺	+	3′-MANT-ATP	Methylpiperazino-FSK	3.27	Mou et al., 2006
2GVZ	363-580	870-1081	Mn²⁺	GTPγS	3′-MANT-ATP	Methylpiperazino-FSK	3.27	Mou et al., 2006
3C14	5C1 (dog)	2C2 (rat)	—	+	PP	FSK	2.68	Mou et al., 2009
3C14	363-580	870-1081	Ca²⁺	GTPγS	PP	FSK	2.68	Mou et al., 2009
3C15	5C1 (dog)	2C2 (rat)	—	+	PP	FSK	2.78	Mou et al., 2009
3C15	363-580	870-1081	Mg²⁺	GTPγS	PP	FSK	2.78	Mou et al., 2009
3C16	5C1 (dog)	2C2 (rat)	Ca²⁺	+	ATP	FSK	2.87	Mou et al., 2009
3C16	363-580	870-1081	Ca²⁺	GTPγS	ATP	FSK	2.87	Mou et al., 2009
3G82	5C1 (dog)	2C2 (rat)	Mn²⁺	+	3′-MANT-ITP	FSK	3.11	Hübner et al., 2011a
3G82	363-580	870-1081	Mn²⁺	GTPγS	3′-MANT-ITP	FSK	3.11	Hübner et al., 2011a
3MAA	5C1 (dog)	2C2 (rat)	—	+	Adenosine-5′-RP-(α-thio-PPP)	Methylpipera-zino-FSK	3.00	Mou et al., 2009
3MAA	363-580	870-1081	Ca²⁺	GTPγS	Adenosine-5′-RP-(α-thio-PPP)	Methylpipera-zino-FSK	3.00	Mou et al., 2009

P, monophosphate; PP, pyrophosphate; PPP, triphosphate; 3′-MANT, 3′-O-(N-methylanthraniloyl)-; TNP, 2′,3′-O-(2,4,6-trinitrophenyl)-.
↵^a Metal ions A and B located in the nucleotide binding site.
↵^b Bovine Gα_s not present (−) or present (+) as chain C in the complex together with GTPγS and mostly metal and Cl⁻ ions (not shown).
↵^c Resolution [Å].
↵^d Homodimer of 2C2.