TABLE 8

Interaction of prototypical membrane-permeable sAC inhibitors and the membrane-impermeable P-site inhibitor 2’,5′-dd-3′-ATP with sAC and mACs

pIC₅₀ values are listed. Structures of prototypical sAC inhibitors and 2′,5′-dideoxy-3′-ATP are shown in Figure 15. pIC₅₀ values for ACs 3, 4 and 6 and sGC are not available. 2′,5′-dideoxy-3′-ATP is mechanistically related to the P-site inhibitors documented in Fig. 13 and Table 7.

AC	2CE	KH7	RU-0204277	2’,5′-dideoxy-3′-ATP
Purified sAC	∼5.7^a	5.52^b	∼5.0^c	6.16^d
5C1:2C2	N.D.	N.D.	N.D.	7.42^d
mAC lysate	∼4^a	no inhibition at 500 μM^b	N.D.	7.40^d
AC1	∼4^a	N.D.	no inhibition at 50 μM^c	7.43^d
AC2	∼4^a	N.D.	no inhibition at 50 μM^c	6.66^d
AC5	∼4^a	N.D.	no inhibition at 50 μM^c	7.43^d
AC7	∼5.7^a	N.D.	N.D.	N.D.
AC8	N.D.	N.D.	no inhibition at 50 μM^c	N.D.
AC9	N.D.	N.D.	no inhibition at 50 μM^c	N.D.

N.D., not determined. Specific experimental conditions for analysis of inhibitors varied in the different studies and are given in:
↵^a Steegborn et al. (2005);
↵^b Bitterman et al. (2013);
↵^c Ramos-Espiritu et al. (2016);
↵^d Gille et al. (2004).