TABLE 1

Fluorogenic peptides

SubunitPreferenceSubstrate
β1AcidicAc-nLPnLD-amc
Z-LLE-amc
β1iHydrophobicAc-PAL-amca
β2BasicBoc-LRR-amc
Ac-RLR-amc
β2iBasicNot applicableb
β5HydrophobicSuc-LLVY-amc
Ac-WLA-amc
β5iHydrophobicAc-ANW-amca

  • Ac-nLPnLD-aminoluciferin, N-acetyl-norleucinal-proline-norleucinal-aspartate-amc; Boc-LRR, tert-butoxycarbonyl-leucine-arginine-arginine.

  • ↵a These substrates differentiate between β5 and β5i activity.

  • ↵b Substrates specific for β2i have not been developed to date.