TABLE 1

Contacts between apelin-13 and the apelin receptor, inferred from the crystal structure PDBID 5VBL

Apelin-13 Contact	Receptor Contacts	References
Q1 Sidechain	D92^{^a}	Gerbier et al., 2015
Q1 Backbone	N177
P3 Sidechain	T22
P3 Sidechain	D23^{^a}	Zhou et al., 2003a
R4 Backbone	D23^{^a}	Zhou et al., 2003a
L5 Sidechain	E20^{^a}	Zhou et al., 2003a
L5 Sidechain	T22
S6 Sidechain	E174^{^a}	Chapman et al., 2014; Gerbier et al., 2015
S6 Backbone	Y21
H7 Sidechain	E174^{^a}	Chapman et al., 2014; Gerbier et al., 2015
K8 Sidechain	Y21	Gerbier et al., 2015
	D284^{^a}	Ma et al., 2017
	S275
G9 backbone	Y271
P10 Sidechain	E198^{^a}
P10 Sidechain	L173	Chapman et al., 2014
M11 Sidechain	W24
	Y271^{^a}	Ma et al., 2017
	F291
	K268^{^b}	Kumar et al., 2016
P12 Sidechain	W24
P12 Sidechain	Y93
P12 Backbone	R168^{^a}	Ma et al., 2017
F13 Sidechain	W85^{^a}
	Y88
	T89
	Y93
	Y299
F13 Carboxylate	K268^{^b}	Kumar et al., 2016
F13 Carboxylate	Y264

↵^a Receptor residues implicated in apelin binding by mutagenesis.
↵^b Receptor residues affecting bias and internalization by mutagenesis.