TABLE 5

Modeling of tertiary structures of OCTs and hMATE2-K

The analysis of homology was performed with program EMBOSS Needle (EMBL-EBI).

Transporter (Modeled Conformation)Crystallized Model Transporter (Conformational)Homology a.a. Identity (a.a. Similarity)ProcedureReferences
rOCT1 (i-open)Lactose permease LacY from E. coli (i-open)14.8% (23.0%)Crystal structure–based homology modelingAbramson et al., 2003; Popp et al., 2005
rOCT1 (o-open)Based on prediction of structural changes of modeled i-open stateVolk et al., 2009
hOCT1 (i-open)Glycerol-3-phosphate transporter GlpT from E. coli (i-open)15.0% (25.5%)Crystal structure–based homology modelingHuang et al., 2003; Boxberger et al., 2018
hOCT1 (o-open)Several members of the MFSIntegrative combinational modelingRoy et al., 2010; Dakal et al., 2017
rOCT2 (i-open)LacY from E. coli (i-open)14.0% (24.0%)Crystal structure–based homology modelingAbramson et al., 2003; Schmitt et al., 2009
rabbit OCT2 (i-open)Lactose permease LacY and glycerol-3-phosphate transporter GlpT from E. coli (i-open)14.4% (22.3%) 10.5% (20.0%)Crystal structure–based homology modelingAbramson et al., 2003; Huang et al., 2003; Zhang et al., 2005
hOCT2 (i-open)Lactose permease LacY and glycerol-3-phosphate transporter GlpT from E. coli (i-open)13.4% (23.2%) 14.6% (26.2%)Crystal structure–based homology modelingAbramson et al., 2003; Huang et al., 2003; Pelis et al., 2006
hOCT3 (i-open)Glycerol-3-phosphate transporter GlpT from E. coli (i-open)15.3% (26.4%)Crystal structure–based homology modelingHuang et al., 2003; Chen et al., 2010a
mOCT3 (o-open)Human glucose transporter GLUT3 (o-occ)19.5% (34.2%)Crystal structure–based homology modelingDeng et al., 2015; Song et al., 2019
hMATE2-K (o-open)MATE transporter NorM from vibrio cholerae (o-open)17.6% (34.4%)Crystal structure–based homology modelingHe et al., 2010; Choi et al., 2011
  • a.a, amino acid; i-open, inward open conformation; o-occ, outward occluded conformation; o-open, outward open conformation.