TABLE 5

Ontogeny profile of hepatic efflux transporters in rats based on efflux activity, protein expression, and mRNA expression levels

Percentages represent expression/activity relative to adult levels.

Onset of Activity and/or ExpressionAdult Levels ReachedAge-Related Changes in Activity/ExpressionCommentsReferences
BSEP
 Protein expressionFetal development: <3%28 days: 78%Increased slowlyAdult age: NR. Strain and sex: Sprague Dawley (F). Methods: Western blottingTomer et al. (2003)
 mRNA expressionFetal development: <10%21 daysIncreased progressivelyAdult age: 60 days. Strain and sex: Sprague Dawley (F) and Wistar (M/F). Methods: RT-PCR, Northern blottingZinchuk et al. (2002); Tomer et al. (2003); St-Pierre et al. (2004)
MRP1
 mRNA expressionFetal development: 300%–500%Fetal developmentDecreased rapidly (14 days: 200%)Adult age: 60 days. Strain and sex: Sprague Dawley and Wistar (M/F). Methods: RT-PCRSt-Pierre et al. (2004); Zhu et al. (2017)
MRP2
 Protein expressionBirth (50%–60%)M: 5 days; F: 35 days (73%)M: increased rapidly; F: increased slowlyAdult age: 35 days. Fetal development: NR. Strain and sex: Sprague Dawley (M/F). Methods: Western blotting.Johnson et al. (2002); Tomer et al. (2003)
 mRNA expressionFetal development (M: 60%, F: 100%)M: 1 days; F: fetal developmentInconsistent patternAdult age: 60–70 days. Strain and sex: Sprague Dawley and Wistar (M/F). Methods: RT-PCR, Northern blotting, branched DNA signal amplification assayJohnson et al. (2002); Zinchuk et al. (2002); Tomer et al. (2003); St-Pierre et al. (2004); Zhu et al. (2017)
MRP3
 mRNA expressionFetal development (M: 60%, F: 10%)M: 1 days; F: 35 daysM: no changes; F: increased progressively. Decreased in elderlyAdult age: 60 days. Strain and sex: Sprague Dawley and Wistar (M/F). Methods: RT-PCRSt-Pierre et al. (2004); Zhu et al., (2017)
MRP4
 mRNA expressionFetal development (M: <30%, F: 200%)M: 28 days; F: fetal developmentM: decreased in elderly (540 days: 40%); F: no changesAdult age: 60 days. Strain and sex: Sprague Dawley (M/F). Methods: RT-PCRZhu et al. (2017)
MRP6
 mRNA expressionFetal development: <20%Birth: 40%NRBirth–adult: NR. Adult age: NR. Strain and sex: Wistar (M/F). Methods: RT-PCRSt-Pierre et al. (2004)
BCRP
 mRNA expressionFetal development (M: 200%, F: 100%)Fetal developmentInconsistent patternAdult age: 60–70 days. Strain and sex: Sprague Dawley (M/F) and Wistar (M). Methods: RT-PCRKawase et al. (2015); Zhu et al. (2017)
  • F, female; M, male; NR, not reported; RT-PCR, reverse-transcriptase polymerase chain reaction.