Onset of Activity and/or Expression | Adult Levels Reached | Age-Related Changes in Activity/Expression | Comments | References | |
---|---|---|---|---|---|
BSEP | |||||
Protein expression | Fetal development: <3% | 28 days: 78% | Increased slowly | Adult age: NR. Strain and sex: Sprague Dawley (F). Methods: Western blotting | Tomer et al. (2003) |
mRNA expression | Fetal development: <10% | 21 days | Increased progressively | Adult age: 60 days. Strain and sex: Sprague Dawley (F) and Wistar (M/F). Methods: RT-PCR, Northern blotting | Zinchuk et al. (2002); Tomer et al. (2003); St-Pierre et al. (2004) |
MRP1 | |||||
mRNA expression | Fetal development: 300%–500% | Fetal development | Decreased rapidly (14 days: 200%) | Adult age: 60 days. Strain and sex: Sprague Dawley and Wistar (M/F). Methods: RT-PCR | St-Pierre et al. (2004); Zhu et al. (2017) |
MRP2 | |||||
Protein expression | Birth (50%–60%) | M: 5 days; F: 35 days (73%) | M: increased rapidly; F: increased slowly | Adult age: 35 days. Fetal development: NR. Strain and sex: Sprague Dawley (M/F). Methods: Western blotting. | Johnson et al. (2002); Tomer et al. (2003) |
mRNA expression | Fetal development (M: 60%, F: 100%) | M: 1 days; F: fetal development | Inconsistent pattern | Adult age: 60–70 days. Strain and sex: Sprague Dawley and Wistar (M/F). Methods: RT-PCR, Northern blotting, branched DNA signal amplification assay | Johnson et al. (2002); Zinchuk et al. (2002); Tomer et al. (2003); St-Pierre et al. (2004); Zhu et al. (2017) |
MRP3 | |||||
mRNA expression | Fetal development (M: 60%, F: 10%) | M: 1 days; F: 35 days | M: no changes; F: increased progressively. Decreased in elderly | Adult age: 60 days. Strain and sex: Sprague Dawley and Wistar (M/F). Methods: RT-PCR | St-Pierre et al. (2004); Zhu et al., (2017) |
MRP4 | |||||
mRNA expression | Fetal development (M: <30%, F: 200%) | M: 28 days; F: fetal development | M: decreased in elderly (540 days: 40%); F: no changes | Adult age: 60 days. Strain and sex: Sprague Dawley (M/F). Methods: RT-PCR | Zhu et al. (2017) |
MRP6 | |||||
mRNA expression | Fetal development: <20% | Birth: 40% | NR | Birth–adult: NR. Adult age: NR. Strain and sex: Wistar (M/F). Methods: RT-PCR | St-Pierre et al. (2004) |
BCRP | |||||
mRNA expression | Fetal development (M: 200%, F: 100%) | Fetal development | Inconsistent pattern | Adult age: 60–70 days. Strain and sex: Sprague Dawley (M/F) and Wistar (M). Methods: RT-PCR | Kawase et al. (2015); Zhu et al. (2017) |
F, female; M, male; NR, not reported; RT-PCR, reverse-transcriptase polymerase chain reaction.