Distribution of α6-subunits in the nervous system and in peripheral tissues
| Region | Experimental Subjects | Detection Method | Reference |
|---|---|---|---|
| Central nervous system | |||
| Cerebellum | Human post-mortem study (schizophrenia and control) | RT-qPCR | Bullock et al. (2008) |
| Rats (Long Evans) | RT-qPCR | Bullock et al. (2009) | |
| Rats (undefined) | ISH | Laurie et al. (1992) | |
| Rats (Albino) | ISH | Persohn et al. (1992) | |
| Rats (Spraque-Dawley) | Northern blot | Mhatre and Ticku (1992) | |
| Rats (Spraque-Dawley) | IHC | Pirker et al. (2000) Gutierrez et al. (1996) | |
| Mice (C57BL/6J) | ISH | Varecka et al. (1994) Lein et al. (2007) | |
| α6−/− Mice (C57BL/6) | LacZ-Gabra6 | Jones et al. (1997) | |
| Mice, chicken, and fish | ISH Autoradiography | Bahn et al. (1996) | |
| Cochlear nuclei/ Cochlear root nuclei | Rats (Wistar) | ISH | Campos et al. (2001) |
| Rats (Sprague-Dawley) | IHC | Gutierrez et al. (1996) | |
| Mice (C57BL/6J) | ISH | Varecka et al. (1994) Lein et al. (2007) | |
| α6−/− Mice (C57BL/6) | LacZ-Gabra6 | Jones et al. (1997) | |
| Rats (Wistar) | RT-PCR | Gomez-Nieto et al. (2008) | |
| Cortex (prefrontal) | Human post-mortem study (AUD and control) | RT-qPCR | Gatta et al. (2021) |
| Rats (Long Evans) | RT-qPCR | Agrawal and Dwivedi (2020) | |
| Cortex (superior frontal) | Human post-mortem study (autism and control) | RT-qPCR Western blot | Fatemi et al. (2014) |
| Hippocampus | Rats (Sprague-Dawley) | RT-qPCR, IHC, Western blot | Yang et al. (2016) |
| Inferior colliculus (dorsal) | α6−/− Mice (C57BL/6) | LacZ-Gabra6 | Jones et al. (1997) |
| Infralimbic area | Mice (C57BL/6J) | ISH | Lein et al. (2007) |
| Olfactory nerve | Rats (Sprague-Dawley) | IHC | Gutierrez et al. (1996) |
| Olfactory cortex (peduncular area, taenia tecta) | Mice (C57BL/6J) | ISH | Lein et al. (2007) |
| Orbital layer | Mice (C57BL/6J) | ISH | Lein et al. (2007) |
| Periaqueductal gray | Alcol P rats (Wistar) | RNA seq | McClintick et al. (2016) |
| Parabrachial nucleus | Mice (C57BL/6J) | ISH | Lein et al. (2007) |
| Raphe nucleus (dorsal) | Alcol P rats (Wistar) | RNA seq | McClintick et al. (2015) |
| Retina (inner plexiform layer) | Rats (Sprague-Dawley) | IHC | Gutierrez et al. (1996) |
| Spinal cord | Rats (Sprague-Dawley) (dorsal horn, layer II) | IHC | Gutierrez et al. (1996) |
| Turtles (motor neurons) | RT-PCR, IHC, Western blot | Gomez-Nieto et al. (2008) Andres et al. (2014) | |
| Rats (Sprague-Dawley) (Vc-C1) | IHC Western blot | Kramer and Bellinger (2013) Kramer and Bellinger (2014) | |
| Striatum (ventral) | Mice (C57BL/6J) D3R−/− mice | RT-PCR | Leggio et al. (2015) |
| Substantia nigra | α6−/− Mice (C57BL/6) | LacZ-Gabra6 | Jones et al. (1997) |
| Thalamus (geniculate nuclei) | α6−/− Mice (C57BL/6) | LacZ-Gabra6 | Jones et al. (1997) |
| Peripheral nervous system | |||
| Cochlea | Mice (CBA/J) | RT-PCR | Drescher et al. (1993) |
| Rats (Wistar) (spiral ganglion and organ) | IHC | Yamamoto et al. (2002) | |
| Trigeminal ganglia | Rats (Sprague-Dawley) | IHC and RT-PCR | Hayasaki et al. (2006) |
| Rats (Sprague-Dawley) | IHC and qPCR | Puri et al. (2011) | |
| Rats (Sprague-Dawley) | IHC Western blot | Puri et al. (2012) Kramer and Bellinger (2013) Kramer and Bellinger (2014) | |
| Rats (Wistar) | IHC | Fan et al. (2018) | |
| Rats (Wistar) | RNA seq | Kogelman et al. (2017) | |
| Mice (ICR) | IHC | Tzeng et al. (2021) | |
| Vestibular end-organs | Rats (Sprague-Dawley) | RT-PCR | Rezaee et al. (1999) |
| Peripheral tissues | |||
| T lymphocytes | Human blood samples | RT-qPCR | Dionisio et al. (2011) |
| Jurkat cells (human T lymphocyte cell line) | RT-qPCR | Mendu et al. (2012) | |
| RT-qPCR | Alam et al. (2006) | ||
| Rats (BB strain) (CD4+ and CD8+) | RT-qPCR | Mendu et al. (2011) | |
| Rats (Wistar) (CD4+ and CD8+) | RT-qPCR Western blot | Mendu et al. (2012) | |
| Liver | Human resections | IHC | Bautista et al. (2014) |
| Lung alveolar (type I and II) | Rats (Sprague-Dawley) | RT-qPCR | Jin et al. (2006) |
| Pancreatic α-cells | Rats (Wistar) | RT-qPCR | Jin et al. (2013) |
| Placenta and uterus | Rats (Wistar) | RT-PCR | Akinci and Schofield (1999) |
α6−/− mice, GABAA receptor α6 subunit knockout mice; Alcol P rats, alcohol-preferring rats; autoradiography, [3H]Ro15-4513; AUD, alcohol use disorder; BB strain, congenic Biobreeding strain; CBA/J, a strain of Agouti mice; CD4+: CD4+ T lympocytes; CD8+: CD8+ T lymphocytes; D3R−/− mice, dopamine D3 receptor knockout mice; ICR, Institute of Cancer Research; IHC, immunohistochemistry; ISH, in situ hybridization; LaZ-Gabra6, LacZ expression driven from the Gabra6 locus detected by β-galactosidase staining; Northern blot, Northern blotting for mRNA; PCR, polymerase chain reaction; qPCR, quantitative PCR; RNA seq, RNA sequencing; RT-PCR, reverse-transcription PCR; RT-qPCR, quantitative reverse-transcription PCR; Vc-C1, trigeminal nucleus caudalis and upper cervical region; Western blot, Western blotting for protein.