TABLE 3

Published structures with RAMPs Asterisk (*) indicates structure obtained by cryo-electron microscopy. Otherwise, structures obtained by X-ray crystallography. All structures marked Full include the ECD and TM domains of the RAMP, with no or very poor C-terminal density observed. Stabilizing mutations made to the RAMP and/or GPCR are not listed but may be present.

GPCRRAMPFull length?Relevant molecules presentOther molecules presentPDB RefRef
NoneRAMP1ECD onlyMSE2YX8Kusano et al. (2008)
NoneRAMP2ECD onlyCa+22XVTNot published (Quigley, 2010)
NoneRAMP2ECD onlyMSE3AQEKusano et al. (2012)
CALCRLRAMP1ECD (both RAMP and GPCR)Olcegepant3N6, 3N7, sulfate ion3N7Ster Haar et al. (2010)
CALCRLRAMP1MBP–RAMP1 ECD–CALCRL ECD fusionAM variantMaltose5V6YBooe et al. (2018)
CALCRLRAMP1MBP–RAMP1 ECD–CALCRL ECD fusionAM2Maltose, sodium ion6D1URoehrkasse et al. (2018)
CALCRLRAMP1ECD + TM (no C terminus), CALCRL full lengthCGRP, Gs heterotrimerNb356E3Y*Liang et al. (2018a)
CALCRLRAMP1ECD (both RAMP and GPCR)Telcagepant3N6, N7R3N7Rter Haar et al. (2010)
CALCRLRAMP1ECD (both RAMP and GPCR)(unliganded)sulfate ion, MSE3N7Pter Haar et al. (2010)
CALCRLRAMP1MBP–RAMP1 ECD–CALCRL ECD fusionCGRP analogMaltose, magnesium ion4RWGBooe et al. (2015)
CALCRLRAMP1ECD (both RAMP and GPCR)Erenumab (Fab)6UMGGarces et al. (2020)
CALCRLRAMP1MBP–RAMP1 ECD–CALCRL ECD fusionOlcegepantSN6, tetraethyl glycol (PG4), maltose, alpha-D-glucopyranose6ZISBucknell et al. (2020)
CALCRLRAMP1MBP–RAMP1 ECD–CALCRL ECD fusionHTL22562tetraethyl glycol (PG4), maltose, alpha-D-glucopyranose6ZHOBucknell et al. (2020)
CALCRLRAMP1MBP–RAMP1 ECD–CALCRL ECD fusionHTL0028125 (macrocycle)Unknown (PDB entry on hold)7P0FCansfield et al. (2022)
CALCRLRAMP1MBP–RAMP1 ECD–CALCRL ECD fusionMacrocycle compound 13Unknown (PDB entry on hold)7P0ICansfield et al. (2022)
CALCRLRAMP1ECD + TM (no C terminus), CALCRL full lengthDetergent micelle7KNT*Josephs et al. (2021)
CALCRLRAMP1ECD + TM (no C terminus), CALCRL full lengthCGRPDetergent micelle7KNU*Josephs et al. (2021)
CALCRLRAMP2ECD onlyMSE3AQFKusano et al. (2012)
CALCRLRAMP2MPB-RAMP2 ECD–CALCRL ECD fusionAMMaltose, 1,2-ethanediol4RWFBooe et al. (2015)
CALCRLRAMP2MPB–RAMP2 ECD–CALCRL ECD fusionhigh-affinity AM (37-52) S45R/K46L/S48G/Q50Walpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, maltose, formic acid, amino group6V2EBooe et al. (2020)
CALCRLRAMP2FullAM, Gs heterotrimerNb356UUN*Liang et al. (2020a)
CALCRLRAMP3FullAM2, Gs heterotrimerNb356UVA*Liang et al. (2020a)
CALCRLRAMP3FullAM, Gs heterotrimerNb356UUS*Liang et al. (2020a)
CALCRRAMP1FullRat amylin, Gs heterotrimerNb35, P42, phosphatidylethanolamine, cholesterol hemisuccinate, palmitic acid, 2-acetamido-2-deoxy-beta-D-glucopyranose7TYF*Cao et al. (2022)
CALCRRAMP1FullSalmon CT, Gs heterotrimerNb35, cholesterol hemisuccinate, palmitic acid, 2-acetamido-2-deoxy-beta-D-glucopyranose7TYW*Cao et al. (2022)
CALCRRAMP2FullRat amylin, Gs heterotrimerNb35, cholesterol hemisuccinate, palmitic acid, 2-acetamido-2-deoxy-beta-D-glucopyranose7TYX*Cao et al. (2022)
CALCRRAMP2FullSalmon CT, Gs heterotrimerNb35, cholesterol hemisuccinate, palmitic acid, 2-acetamido-2-deoxy-beta-D-glucopyranose7TYY*Cao et al. (2022)
CALCRRAMP2FullCT, Gs heterotrimerNb357TYH*Cao et al. (2022)
CALCRRAMP3FullRat amylin, Gs heterotrimerNb35, P42, cholesterol hemisuccinate, palmitic acid, 2-acetamido-2-deoxy-beta-D-glucopyranose7TZF*Cao et al. (2022)

  • MBP, maltose binding protein; MSE, selenomethionine, an L-peptide linking molecule; Nb35, Nanobody 35.