TABLE 1

Reports of GPCRs tested for RAMP interaction, followed by summary statistics GPCRs are grouped by family and sorted alphabetically within each family. Key references are provided.

GPCR	Abbreviation	Uniprot	Family	RAMP
Adhesion G-protein coupled receptor F5	ADGRF5	Q8IZF4	Adhesion	RAMP3 (Lorenzen et al., 2019)
Cadherin EGF LAG seven-pass G-type receptor 2	CELSR2	Q9HCU4	Adhesion	None (only RAMP2 tested) (Barbash et al., 2019)
Frizzled family receptor 1	FZD1	Q9UP38	Frizzled	None (only RAMP2 tested) (Barbash et al., 2019)
Calcium-sensing receptor	CaSR	P41180	Glutamate	RAMP1 and 3 (Bouschet et al., 2008; Bouschet et al., 2005; Desai et al., 2014)
Pituitary adenylate-cyclase activating polypeptide type 1	ADYCAP1R1	P41586	Secretin	RAMP1, 2, and 3 (Lorenzen et al., 2019) RAMP2 and 3 (M. Harris et al., preprint, DOI: https://doi.org/10.1101/2021.04.08.436756)
Calcitonin receptor-like receptor	CALCRL	Q16602	Secretin	RAMP1, 2 and 3 (McLatchie et al., 1998) and many others (including solved structures)
Calcitonin receptor	CALCR	P30988	Secretin	RAMP1, 2 and 3 (Armour et al., 1999; Christopoulos et al., 1999) and many others
Corticotropin-releasing hormone receptor 1	CRHR1	P34998	Secretin	None (Tasma et al., 2020) RAMP2 (Wootten et al., 2013; Wootten et al., 2013;Bailey et al., 2019) RAMP3 (Lorenzen et al., 2019) RAMP2,3 (M. Harris et al., preprint)
Corticotropin-releasing hormone receptor 2	CRHR2	Q13324	Secretin	None (Lorenzen et al., 2019; Tasma et al., 2020) None (only RAMP2 tested) (Bailey et al., 2019) RAMP2,3 (M. Harris et al., preprint)
Glucagon receptor	GCGR	P47871	Secretin	RAMP2 (Christopoulos et al., 2003; Weston et al., 2015; Cegla et al., 2017; McGlone et al., 2021) RAMP1,3 (Lorenzen et al., 2019) RAMP1,2,3 (Shao et al., 2022; M. Harris et al., preprint)
Growth hormone-releasing hormone	GHRHR	Q02643	Secretin	None (Christopoulos et al., 2003) RAMP2,3 (Lorenzen et al., 2019) RAMP1,2,3 (Shao et al., 2022; M. Harris et al., preprint) RAMP1,2 with splice variant 1 of GHRHR (Shao et al., 2022)
Gastric inhibitory polypeptide receptor	GIPR	P48546	Secretin	RAMP1,2,3 (Lorenzen et al., 2019; Shao et al., 2022; M. Harris et al., preprint)
Glucagon-like peptide 1 receptor	GLP1R	P43220	Secretin	None (Christopoulos et al., 2003; Wootten et al., 2013) RAMP2,3 (Shao et al., 2022) RAMP1,2,3 (Lorenzen et al., 2019) (M. Harris et al., preprint)
Glucagon-like peptide 2 receptor	GLP2R	O95838	Secretin	None (Christopoulos et al., 2003) RAMP1,2,3 (Lorenzen et al., 2019; M. Harris et al., preprint) RAMP3 (Shao et al., 2022)
Parathyroid hormone 1 receptor	PTH1R	Q03431	Secretin	RAMP2 (Christopoulos et al., 2003; Nemec et al., 2022) RAMP1,3 (Lorenzen et al., 2019) RAMP2,3 (M. Harris et al., preprint) RAMP3 (Phelps et al., 2005)
Parathyroid hormone 2 receptor	PTH2R	P49190	Secretin	RAMP3 (Christopoulos et al., 2003) RAMP1,2 (Lorenzen et al., 2019) RAMP1,2,3 (M. Harris et al., preprint)
Secretin receptor	SCTR	P47872	Secretin	RAMP3 (Harikumar et al., 2009) RAMP1,2 (Lorenzen et al., 2019) RAMP1,2,3 (Shao et al., 2022; M. Harris et al., preprint)
VIP and PACAP receptor 1	VIPR1	P32241	Secretin	RAMP1,2,3 (Christopoulos et al., 2003) (M. Harris et al., preprint) RAMP2,3 (Lorenzen et al., 2019)
VIP and PACAP receptor 2	VIPR2	P41587	Secretin	RAMP1,2,3 (Wootten et al., 2013; (M. Harris et al., preprint) RAMP2,3 (Lorenzen et al., 2019)
Atypical chemokine receptor 1	ACKR1	Q16570	Rhodopsin	RAMP1,2,3 (Mackie et al., 2019)
Atypical chemokine receptor 2	ACKR2	O00590	Rhodopsin	RAMP1,3 (Mackie et al., 2019)
Atypical chemokine receptor 3	ACKR3	P25106	Rhodopsin	RAMP2,3 (Lorenzen et al., 2019) RAMP1,2,3 (Mackie et al., 2019)
Atypical chemokine receptor 4	ACKR4	Q9NPB9	Rhodopsin	RAMP2,3 (Mackie et al., 2019)
Atypical chemokine receptor 5	ACKR5	O00421	Rhodopsin	None (Mackie et al., 2019)
Adenosine A2B receptor	ADORA2B	P29275	Rhodopsin	RAMP2 (Barbash et al., 2019)
Beta 2 adrenergic receptor	B2ADR	P07550	Rhodopsin	None (Mackie et al., 2019; Shao et al., 2022; M. Harris et al., preprint)
C-C Chemokine receptor type 1	CCR1	P32246	Rhodopsin	RAMP1,2,3 (Mackie et al., 2019)
C-C Chemokine receptor type 2	CCR2	P41597	Rhodopsin	RAMP2,3 (Mackie et al., 2019)
C-C Chemokine receptor type 3	CCR3	P51677	Rhodopsin	RAMP1,2,3 (Mackie et al., 2019)
C-C Chemokine receptor type 4	CCR4	P51679	Rhodopsin	RAMP1,2,3 (Mackie et al., 2019)
C-C Chemokine receptor type 5	CCR5	P51681	Rhodopsin	None (Lorenzen et al., 2019) RAMP2,3 (Mackie et al., 2019)
C-C Chemokine receptor type 6	CCR6	P51684	Rhodopsin	RAMP1,2,3 (Mackie et al., 2019)
C-C Chemokine receptor type 7	CCR7	P32248	Rhodopsin	None (Lorenzen et al., 2019) RAMP3 (Mackie et al., 2019)
C-C Chemokine receptor type 8	CCR8	P51685	Rhodopsin	RAMP3 (Mackie et al., 2019)
C-C Chemokine receptor type 9	CCR9	P51686	Rhodopsin	RAMP1,3 (Mackie et al., 2019)
C-C Chemokine receptor type 10	CCR10	P46092	Rhodopsin	RAMP1,2,3 (Mackie et al., 2019)
Chemokine-like receptor 1	CMKLR1	Q99788	Rhodopsin	RAMP2,3 (Mackie et al., 2019)
Chemokine C-X3-C receptor 1	CX3CR1	P49238	Rhodopsin	RAMP1,2,3 (Mackie et al., 2019)
C-X-C chemokine receptor type 1	CXCR1	P25024	Rhodopsin	RAMP1,2 (Mackie et al., 2019)
C-X-C chemokine receptor type 2	CXCR2	P25025	Rhodopsin	RAMP2,3 (Mackie et al., 2019)
C-X-C chemokine receptor type 3	CXCR3	P49682	Rhodopsin	None (Lorenzen et al., 2019) RAMP3 (Mackie et al., 2019)
C-X-C chemokine receptor type 4	CXCR4	P61073	Rhodopsin	None (Lorenzen et al., 2019) RAMP1,3 (Mackie et al., 2019)
C-X-C chemokine receptor type 5	CXCR5	P32302	Rhodopsin	None (Mackie et al., 2019)
C-X-C chemokine receptor type 6	CXCR6	O00574	Rhodopsin	RAMP3 (Mackie et al., 2019)
Proteinase-activated receptor 2	F2RL1	P55085	Rhodopsin	None (only RAMP2 tested) (Barbash et al., 2019)
Proteinase-activated receptor 2	F2RL3	Q96RI0	Rhodopsin	RAMP2 (Barbash et al., 2019)
G-protein coupled receptor 4	GRP4	P46093	Rhodopsin	RAMP1,2,3 (Lorenzen et al., 2019)
G protein-coupled estrogen receptor 1	GPR30	Q99527	Rhodopsin	RAMP3 (Lenhart et al., 2013)
Melatonin-related receptor	GPR50	Q15385	Rhodopsin	None (only RAMP2 tested) (Barbash et al., 2019)
Probably G protein-coupled receptor 141	GPR141	Q7Z602	Rhodopsin	RAMP2 (Barbash et al., 2019)
Probably G protein-coupled receptor 141	GPR160	Q9UJ42	Rhodopsin	None (only RAMP2 tested) (Barbash et al., 2019)
G protein-coupled receptor 176	GPR176	Q80WT4	Rhodopsin	None (only RAMP2 tested) (Barbash et al., 2019)
G-protein coupled receptor 182	GRP182	O15218	Rhodopsin	RAMP1,2,3 (Lorenzen et al., 2019)
Leucine-rich repeat-containing G-protein coupled receptor 4	LGR4	Q9BXB1	Rhodopsin	None (only RAMP2 tested) (Barbash et al., 2019)
P2Y purinoceptor 8	P2RY8	Q86VZ1	Rhodopsin	RAMP2 (Barbash et al., 2019)
Neurotensin receptor type 1	NTSR1	P30989	Rhodopsin	None (only RAMP2 tested) (Barbash et al., 2019)
δ-type opioid receptor	OPRD1	P41143	Rhodopsin	RAMP2 (Barbash et al., 2019)
Sphingosine 1-phosphate receptor 1	S1PR1	P21453	Rhodopsin	None (only RAMP2 tested) (Barbash et al., 2019)
Chemokine XC receptor 1	XCR1	P46094	Rhodopsin	None (Mackie et al., 2019)

Summary statistics	RAMP1	RAMP2	RAMP3
No. of GPCR interactors (at least one study)	28	30	41
	Secretin	Rhodopsin	Glutamate	Adhesion	Frizzled
No. of GPCRs that interact with any RAMP(s) (based on at least one report)	15	29	1	1	—
No. of GPCRs that don’t interact with any RAMP (based on at least one report)	5	8^a	—	1	1
No. of GPCRs in the family	15	719	22	33	11

Key references provided.
^aAnd 7 additional instances where the GPCR does not appear to interact with a RAMP, but only RAMP2 was tested.