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Quantification of α1-Adrenoceptor Subtypes in Human Tissues by Competitive RT-PCR Analysis

https://doi.org/10.1006/bbrc.1995.2219Get rights and content

Abstract

α1-Adrenoceptors are a heterogeneous subfamily of receptors comprising at least three pharmacologically- and structurally- distinct subtypes named α1a-, α1b- and α1d -adrenoceptors. A competitive reverse transcription-polymerase chain reaction methodology using subtype selective competitor RNAs as reference internal standards was adopted to determine the absolute amounts of each of the three cloned to date α1-adrenoceptor subtype encoding mRNAs in selected human tissues. Our data demonstrated that each of these α1-adrenoceptor subtypes exhibits a distinctive tissular expression pattern.

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