Regular Article
Gene Regulation of HBP 23 by Metalloporphyrins and Protoporphyrin IX in Liver and Hepatocyte Cultures

https://doi.org/10.1006/bbrc.1997.6166Get rights and content
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Abstract

Heme-binding protein 23 kDa (HBP23) belongs to the antioxidant family of peroxiredoxins and binds heme with high affinity.In vivotreatment of rats with heme induced expression of HBP23 mRNA levels in liver coordinately with that of the heme degrading enzyme heme oxygenase-1 (HO-1). In primary rat hepatocyte cultures Sn-, Co-, and Zn-metalloprotoporphyrin as well as the heme precursor protoporphyrin IX increased the HBP23 mRNA expression to a level similar to that elicited by heme. Heme-dependent induction of HBP23 mRNA was prevented by pretreatment with actinomycin D, indicating a transcriptional mechanism of gene induction. The results suggest that the coordinate gene regulation pattern of HBP23 and HO-1 plays a physiological role against oxidative stress.

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This study was supported by NIH Grants DK 30203 and 30664 (U.M.E.), the Children's Blood Foundation (U.M.E.), and Grant Im 20/2-1 from the Deutsche Forschungsgemeinschaft, Germany (S.I.).

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Present addresses: Zentrum Innere Medizin, Abt. Gastroenterologie u. Endokrinologie, Georg-August Universität, Göttingen, Germany.

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Present addresses: Department of Biochemistry, Nippon Medical School, 1-1-5 Sendagi Bunkyo-ku, Tokyo, Japan.

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Corresponding author. Departments of Pediatrics Hematology/Oncology, Cornell University Medical College, 525 East 68 Street, Room N-415, New York, NY 10021. Fax: 212-746-8609.