Regular ArticleMolecular Cloning and Expression of Human cGMP-Binding cGMP-Specific Phosphodiesterase (PDE5)☆,☆☆,★
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The nucleotide sequence reported in this paper has been submitted to the GenBank/EMBL Data Bank with Accession Number AJ004865.
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Abbreviations: AA, amino acid; bp, base pair; Da, Dalton; EDTA, ethylene diamine tetraacetic acid; HEPES, N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonate); PAGE, polyacrlyamide gel electrophoresis; PCR, polymerase chain reaction; PDE, phosphodiesterase; SSC, saline sodium citrate buffer; SDS, sodium dodecyl sulfate
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Murad, P.
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Corresponding author. Fax: +44 (0)1304 615600. E-mail:[email protected].