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Molecular Cloning and Expression of Human cGMP-Binding cGMP-Specific Phosphodiesterase (PDE5),☆☆,

https://doi.org/10.1006/bbrc.1998.8769Get rights and content

Abstract

A human PDE5 cDNA has been isolated which contains an open reading frame encoding an 875 amino acid, 100,012 Da polypeptide, the expression of which yields a protein of the predicted size and is capable of hydrolyzing cGMP. The deduced amino acid sequence is very similar (95%) to that of bovine PDE5, and comprises a conserved cGMP-binding domain and catalytic domain. Northern analysis reveals a major and minor transcript of ∼9 kb and ∼8 kb respectively, thus indicating the existence of at least two splice variants, the major form being readily detected in bladder, colon, lung, pancreas, placenta, prostate, small intestine, and stomach.

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    The nucleotide sequence reported in this paper has been submitted to the GenBank/EMBL Data Bank with Accession Number AJ004865.

    ☆☆

    Abbreviations: AA, amino acid; bp, base pair; Da, Dalton; EDTA, ethylene diamine tetraacetic acid; HEPES, N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonate); PAGE, polyacrlyamide gel electrophoresis; PCR, polymerase chain reaction; PDE, phosphodiesterase; SSC, saline sodium citrate buffer; SDS, sodium dodecyl sulfate

    Murad, P.

    2

    Corresponding author. Fax: +44 (0)1304 615600. E-mail:[email protected].

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