RGS8 Protein Is Distributed in Dendrites and Cell Body of Cerebellar Purkinje Cell

doi:10.1006/bbrc.2001.5489

Biochemical and Biophysical Research Communications

Volume 287, Issue 1, 14 September 2001, Pages 223-228

https://doi.org/10.1006/bbrc.2001.5489 Get rights and content

Abstract

RGS8 was originally identified as an RGS protein specifically expressed in neuronally differentiated P19 cells. We generated a polyclonal antibody specific to rat RGS8 using a synthetic peptide. When nonneural cells (DDT1MF2, CHO, and NIH3T3) transfected with rat RGS8 cDNA were immuno-stained with this antibody, the RGS8 protein was mainly detected in the nuclei. Since RGS8 mRNA was exclusively expressed in Purkinje cells of the cerebellum in the rat brain, we further examined the cellular distribution of the RGS8 protein in Purkinje cells using cultured cerebellar cells and tissue sections of the cerebellum. The RGS8 protein was excluded from the nuclei and distributed in the cell body and dendrites, but not in the axons of Purkinje cells. These results demonstrate the presence of a mechanism controlling the distribution of RGS8 protein in cerebellar Purkinje cells.

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Citation Excerpt :
We further observed that co-expression of active Gαo resulted in the translocation of RGS8 to the plasma membrane [11]. On the other hand, when tissue sections of the cerebellum were stained with RGS8 antibody, we found that the RGS8 protein was excluded from nucleus and distributed in the cell body and dendrites [12]. Since Gα may not be constitutively activated in normal unstimulated cells, it was considered that neural cells may express certain protein factors to induce the translocation of RGS8.
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^☆: Abbreviations used: G proteins, heterotrimeric guanine nucleotide-binding proteins; RGS, regulators of G protein signaling.

¹: To whom correspondence should be addressed. Fax: (+81) 423-21-8678. E-mail: [email protected].

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Regular ArticleRGS8 Protein Is Distributed in Dendrites and Cell Body of Cerebellar Purkinje Cell☆

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Regular Article
RGS8 Protein Is Distributed in Dendrites and Cell Body of Cerebellar Purkinje Cell☆