Elsevier

Genomics

Volume 33, Issue 3, 1 May 1996, Pages 374-381
Genomics

Regular Article
Structure and Genomic Organization of the Human B1Receptor Gene for Kinins (BDKRB1)

https://doi.org/10.1006/geno.1996.0213Get rights and content

Abstract

Two subtypes of mammalian bradykinin receptors, B1and B2(BDKRB1 and BDKRB2), have been defined based on their pharmacological properties. The B1type kinin receptors have weak affinity for intact BK or Lys-BK but strong affinity for kinin metabolites without the C-terminal arginine (e.g., des-Arg9-BK and Lys-des-Arg9-BK, also called des-Arg10-kallidin), which are generated by kininase I. The B1receptor expression is up-regulated following tissue injury and inflammation (hyperemia, exudation, hyperalgesia, etc.). In the present study, we have cloned and sequenced the gene encoding human B1receptor from a human genomic library. The human B1receptor gene contains three exons separated by two introns. The first and the second exon are noncoding, while the coding region and the 3′-flanking region are located entirely on the third exon. The exon–intron arrangement of the human B1receptor gene shows significant similarity with the genes encoding the B2receptor subtype in human, mouse, and rat. Sequence analysis of the 5′-flanking region revealed the presence of a consensus TATA box and of numerous candidate transcription factor binding sequences. Primer extension experiments have shown the existence of multiple transcription initiation sites situated downstream and upstream from the consensus TATA box. Genomic Southern blot analysis indicated that the human B1receptor is encoded by a single-copy gene.

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