Elsevier

Genomics

Volume 45, Issue 1, 1 October 1997, Pages 97-104
Genomics

Regular Article
Celsr1, a Neural-Specific Gene Encoding an Unusual Seven-Pass Transmembrane Receptor, Maps to Mouse Chromosome 15 and Human Chromosome 22qter

https://doi.org/10.1006/geno.1997.4892Get rights and content

Abstract

We have identifiedCelsr1,a gene that encodes a developmentally regulated vertebrate seven-pass transmembrane protein. The extracellular domain of Celsr1 contains two regions each with homology to distinct classes of well-characterized motifs found in the extracellular domains of many cell surface molecules. The most N-terminal region contains a block of contiguous cadherin repeats, and C-terminal to this is a region containing seven epidermal growth factor-like repeats interrupted by two laminin A G-type repeats. Celsr1 is unique in that it contains this combination of repeats coupled to a seven-pass transmembrane domain. As part of the characterization of theCelsr1gene, we have determined its chromosomal map location in both mouse and human. The European Collaborative Interspecific Backcross (EUCIB) and BXD recombinant inbred strains were used for mappingCelsr1cDNA clones in the mouse, and fluorescencein situhybridization was used to map humanCelsr1cosmid clones on metaphase chromosomes. We report thatCelsr1maps to proximal mouse Chromosome 15 and human chromosome 22qter, a region of conserved synteny. Reverse transcriptase–polymerase chain reaction analysis andin situhybridization were used to determine the spatial restriction ofCelsr1transcripts in adult and embryonic mice. The results presented here extend our previous finding of expression of the Celsr1 receptor in the embryo and show that expression continues into adult life when expression in the brain is localized principally in the ependymal cell layer, choroid plexus, and the area postrema.

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    C. D. SternP. W. H. Holland, Eds.

    1

    Present address: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto M5G 1X5, Canada.

    2

    To whom correspondence should be addressed. Telephone: 44-171 594 5288. Fax: 44-171 823 7525. E-mail: [email protected].

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