Communication
Dynamic DNA Contacts Observed in the NMR Structure of Winged Helix Protein-DNA Complex,☆☆

https://doi.org/10.1006/jmbi.1999.2819Get rights and content

Abstract

Genesis is an HNF-3/fkh homologous protein. By using multi-dimensional NMR techniques, we have obtained the solution structure and backbone dynamics of Genesis complexed with a 17 base-pair DNA. Our results indicate that both the local folding and dynamic properties of Genesis are perturbed when it binds to the DNA site. Our data show that a conserved flexible amino acid sequence (wing 1) makes dynamic contacts to DNA in the complex and a short helix is induced by Genesis-DNA interactions. Our data indicate that, unlike the HNF-3γ/DNA complex, a magnesium ion is not required in forming the stable Genesis-DNA complex.

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Abbreviations used: HNF, hepatocyte nuclear factor; fkh, forkhead;T1, longitudinal relaxation time constant;T2, transverse relaxation time constant; NOE, nuclear Overhauser effect; TOCSY, total correlated spectroscopy; HSQC, heteronuclear single quantum correlation

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Edited by P. E. Wright

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E-mail address of the corresponding author: [email protected]

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