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Olfactory Receptors, Golfαand Adenylyl Cyclase mRNA Expressions in the Rat Heart During Ontogenic Development

https://doi.org/10.1006/jmcc.1999.0945Get rights and content

Abstract

Golfα(a G heterotrimeric protein which shares a high homology with Gsα) expression was studied in the rat heart before birth and until weaning. Since Golfαin the neuro-olfactory epithelium is coupled to olfactory receptors and type III adenylyl cyclase, we looked for the presence of such molecules in the heart. GolfαmRNA was detected in the rat heart, highest levels being found in 21-day old fetuses until 3 days post partum. The protein amounts measured by Western blots paralleled the GolfαmRNA levels. Immunohistochemical studies revealed the presence of Golfαin atrial and ventricular cardiomyocytes. OL1 and latrophilin mRNAs, G protein-coupled olfactory receptors, were expressed at early postnatal stages. Adenylyl cyclase mRNAs for type II, type III, type V and type VI were expressed before birth and until weaning. Elements for an unexpected signaling pathway involving odorant receptors like OL1 and latrophilin, Golfαand type III adenylyl cyclase were expressed in rat heart, and appeared developmentally regulated.

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    A pattern was likewise emerging for the other components of the olfactory signal transduction pathway. Gαolf was identified as an olfactory-specific G protein (Jones & Reed, 1989), yet transcripts have also been detected the heart and pancreas (Ferrand et al., 1999; Frayon et al., 1999). Moreover, Gαolf has also been reported to be critical in dopamine D1 receptor and adenosine A2a receptor signaling in the basal ganglia and striatum (Herve et al., 1993; Zhuang et al., 2000; Corvol et al., 2001).

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    There are three Gsα isoforms: Gαolf and the Gsα splice variants GsαS and GsαL[4–8]. Gsα proteins are differentially expressed in various organs including heart, liver and brain, and Gsα isoform expression changes during development and aging [9–19]. Additionally, Gsα expression changes in various pathological conditions [20–25].

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Please address all correspondence to: Dr Nathalie Ferrand, Unité 482 de l»INSERM, Hôpital Saint-Antoine, Bâtiment Inserm, 184, rue du Faubourg Saint-Antoine, 75571 Paris-Cedex 12, France.

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