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Homer-1c/Vesl-1L Modulates the Cell Surface Targeting of Metabotropic Glutamate Receptor Type 1α: Evidence for an Anchoring Function

https://doi.org/10.1006/mcne.1999.0808Get rights and content

Abstract

Homer-1c/Vesl-1L is a 48-kDa protein that forms part of a family of conserved Homer-related proteins that interact with the C-termini of the metabotropic glutamate receptors mGluR1α and mGluR5. In order to examine the function of Homer-1c, HEK-293 cells have been transfected with mGluR1α, Homer-1c, and both proteins together. When cells were transfected with both proteins, biotinylation of cell surface molecules revealed a significant increase in the amount of receptor and Homer-1c associated with the cell surface compared with cells transfected with mGluR1α alone. This finding was paralleled by a concomitant increase in the production of inositol after treatment of the doubly transfected cells with agonist. Cell surface immunostaining of mGluR1α showed that Homer-1c can induce clustering of the receptor in the plasma membrane of HEK-293 cells and suggested that the surface receptor was associated with Homer-1c in the plasma membrane. The presence of Homer-1c reduced the rate of loss from the cell surface of mGluR1α from 5 to 1%/min and increased the extent of dendritic trafficking of the receptor in rat primary cultured neurons. Our results suggest that Homer-1c increases the cell surface expression of the metabotropic glutamate receptor type 1α by increasing its retention in the plasma membrane.

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