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RNA pp 29–41Cite as

Synthesis of RNA by In Vitro Transcription

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 703))

Abstract

In vitro transcription is a simple procedure that allows for template-directed synthesis of RNA molecules of any sequence from short oligonucleotides to those of several kilobases in μg to mg quantities. It is based on the engineering of a template that includes a bacteriophage promoter sequence (e.g. from the T7 coliphage) upstream of the sequence of interest followed by transcription using the corresponding RNA polymerase. In vitro transcripts are used in analytical techniques (e.g. hybridization analysis), structural studies (for NMR and X-ray crystallography), in biochemical and genetic studies (e.g. as antisense reagents), and as functional molecules (ribozymes and aptamers).

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Author information

Authors and Affiliations

  1. Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen, Denmark

    Bertrand Beckert

  2. Architecture et Réactivité de l’ARN, Université de Strasbourg, CNRS, IBMC, Strasbourg, France

    Benoît Masquida

Authors
  1. Bertrand Beckert
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  2. Benoît Masquida
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Corresponding author

Correspondence to Benoît Masquida .

Editor information

Editors and Affiliations

  1. The Panum Institute, Dept. of Cellular & Molecular Medicine, University of Copenhagen, Blegdamsvej 3, Copenhagen, 2200N, Denmark

    Henrik Nielsen

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Beckert, B., Masquida, B. (2011). Synthesis of RNA by In Vitro Transcription. In: Nielsen, H. (eds) RNA. Methods in Molecular Biology, vol 703. Humana Press. https://doi.org/10.1007/978-1-59745-248-9_3

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  • DOI: https://doi.org/10.1007/978-1-59745-248-9_3

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-913-0

  • Online ISBN: 978-1-59745-248-9

  • eBook Packages: Springer Protocols

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