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The intestine as source of immunoreactive substance P in plasma of the cat

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Summary

  1. 1.

    Substance P (SP) was measured in acid acetone extracted plasma of cats using a sensitive radioimmunoassay. The immunoreactive material was submitted to ion exchange chromatography and at least 90% of immunoreactivity co-chromatographed with synthetic SP.

  2. 2.

    The level of immunoreactive SP (I-SP) in extracted plasma of the cat was 69.3±9.8 fmol/ml with values ranging from 2.5 to 165 fmol/ml. Evisceration of the cats caused a decrease of I-SP levels from 70.8±30.8 fmol/ml to 20.8±9.9 15 min and to 26.8±19.7 fmol/ml 60 min after the operation.

  3. 3.

    Ligation of intestinal blood vessels led to a fall in I-SP levels from 58.7±11.5 to 25.9±4.1 fmol/ml within 15 min.

  4. 4.

    No difference between I-SP values in portal (71.8±11.2 fmol/ml) and peripheral plasma (68.3±12.1 fmol/ml) was found under the conditions in which these experiments were performed.

  5. 5.

    It is concluded that a major part of circulating immunoreactive SP originates from the intestine.

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Authors and Affiliations

  1. Institut für experimentelle und klinische Pharmakologie, Universitätsplatz 4, A-8010, Graz, Austria

    R. Gamse & F. Lembeck

  2. Laboratory of Human Reproduction and Reproductive Biology, Department of Physiology, Harvard Medical School, 02115, Boston, Massachusetts, U.S.A.

    E. Mroz & S. Leeman

Authors
  1. R. Gamse
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  2. E. Mroz
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  3. S. Leeman
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  4. F. Lembeck
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Additional information

This work was supported by grant No. 3400 of the Fonds zur Förderung der wissenschaftlichen Forschung in Österreich.

Preliminary results were published in Naunyn Schmiedeberg's Arch. Pharmacol. 302 Suppl. R57 (1978)

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Gamse, R., Mroz, E., Leeman, S. et al. The intestine as source of immunoreactive substance P in plasma of the cat. Naunyn-Schmiedeberg's Arch. Pharmacol. 305, 17–21 (1978). https://doi.org/10.1007/BF00497001

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  • DOI: https://doi.org/10.1007/BF00497001

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