Summary
Washed human red cells, suspended in an isotonic electrolyte solution, were incubated with radioactive catecholamines (epinephrine, norepinephrine, dopamine and isoproterenol). Their conversion to 3-O-methyl derivatives was demonstrated by ion exchange chromatography for each substrate. There was no O-methylation if the erythrocytes were boiled or hemolyzed prior to incubation or if the catechol-O-methyl transferase (COMT) inhibitor pyrogallol was added to the incubation medium. Only catecholamines were methylated by this system, as could be demonstrated by using the non-catecholamine orciprenaline as substrate. Experiments with l-methionine-(methyl-14C) showed that the methylation was brought about by the transfer of a methyl group from l-methionine to the 3-hydroxy group of epinephrine. Thus, this enzymatic system had the properties of COMT. Our experiments therefore suggest that COMT is present in human erythrocytes.
Similar content being viewed by others
References
Axelrod, J., Cohn, C. K.: Methyltransferase enzymes in red blood cells. J. Pharmacol. exp. Ther. 175, 650–654 (1971).
—, Tomchick, R.: Enzymatic O-methylation of epineprhine and other catechols. J. biol. Chem. 233, 702–705 (1958).
Bryson, G., Bischoff, F.: Dopamine transport in human blood. Clin. Chem. 16, 312–317 (1970).
Cantoni, G. L.: Preparation of S-adenosyl-methionine. In: Methods in enzymology, vol. 3, p. 600. S. Colowick and N. O. Kaplan, ed. New York: Academic Press 1957.
Cohn, C. K., Dunner, D. L., Axelrod, J.: Reduced catechol-O-methyltransferase activity in red blood cells of women with primary affective disorder. Science 170, 1323–1234 (1970).
Häggendal, J.: On the use of strong exchange resins for determination of small amounts of catechol amines. Scand. J. clin. Lab. Invest. 14, 537–544 (1962).
Horst, W. D., Gattanell, P., Urbano, S., Sheppard, H.: Catechol-O-methyl transferase in erythrocytes and fat cells. Life Sci. 8, 473–476 (1969).
Kopin, I. J., Axelrod, J., Gordon, E.: The metabolic fate of H3-epinephrine and C14-metanephrine in the rat. J. biol. Chem. 236, 2109–2113 (1961).
Kroneberg, G., Schümann, H. J.: Adrenalinsekretion und Adrenalinverarmung der Kaninchennebennieren nach Reserpin. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 234, 133–146 (1958).
Kübler, W., Männl, H. F. K.: Die Permeation von Isoproterenol durch die Erythrocytenmembran des Menschen. Klin. Wschr. 48, 944–946 (1960).
Männl, H. F. K., Hempel, K., Kübler, W.: Über die O-Methylierung von Katecholaminen in Erythrocyten des Menschen. Naunyn-Schmiedebergs Arch. Pharmak. 266, 398 (1970).
Roston, S.: Rapid movement of epinephrine and norephinephrine into human erythrocytes. Nature (Lond.) 215, 432–433 (1967).
Schanker, L. S., Nafpliotis, P. A., Johnson, J. M.: Passage of organic bases into human red cells. J. Pharmacol. exp. Ther. 133, 325–331 (1961).
Wyatt, R. J., Portnoy, B., Kupfer, D. J., Snyder, F., Engelman, K.: Resting plasma catecholamine concentrations in patients with depression anxiety. Arch. gen. Psychiat. 24, 65–70 (1971).
Author information
Authors and Affiliations
Additional information
This investigation was supported by the Deutsche Forschungsgemeinschaft.
Rights and permissions
About this article
Cite this article
Männl, H.F.K., Hempel, K. & Kübler, W. Catechol-O-methyl transferase in human erythrocytes. Naunyn-Schmiedeberg's Arch. Pharmacol. 272, 265–276 (1972). https://doi.org/10.1007/BF00499038
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00499038