Summary
Small intestinal mucosal samples from man, pig and dog, were subjected to sequential or correlative silver impregnation techniques, applied to immunocytochemical preparations and at the ultrastructural level.
The cell reacting with anti-GIP sera was identified as the ultrastructurally classified K cell and we propose that the term GIP cell be used in place of the latter.
This cell can thus be recognized by its strong reactivity with the Sevier-Munger staining procedure, provided that the equally strongly reacting EC cell is excluded by virtue of its argentaffinity with the Masson technique.
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Brown, J. C., Dryburgh, J. R.: A gastric inhibitory polypeptide. II. The complete amino acid sequence. Canad. J. Biochem. 49, 867–872 (1971)
Brown, J. C., Mutt, V., Pederson, R. A.: Further purification of a polypeptide demonstrating enterogastrone activity. Canad. J. Physiol. Pharmacol. 47, 113–114 (1970)
Bussolati, G., Capella, C., Vassallo, G., Solcia, E.: Histochemical and ultrastructural studies on pancreatic A cells. Evidence for glucagon and non-glucagon components of the α granule. Diabetologia 7, 181–188 (1971)
Capella, C., Solcia, E.: The endocrine cells of the pig gastrointestinal mucosa and pancreas. Arch. histol. jap. 35, 1–29 (1972)
Capella, C., Solcia, E., Vassallo, G.: Ultrastructural and histochemical investigations on the endocrine cells of the intestinal mucosa. In: S. Taylor (ed.), Endocrinology 1971, Proc. Int. Symp. p. 282–290. London: Heinemann 1972
Cavallero, C., Vassallo, G., Capella, C., Solcia, E.: Ultrastructure of intestinal endocrine cells in man. In: Advances in gastrointestinal endoscopy, p. 449–455. Padova: Piccin 1972
Erspamer, V.: Caratteristiche delle cellule enterocromaffini tipiche e delle cellule preenterocromaffin argentofile. Anat. Anz. 86, 379 (1938)
Grimelius, L.: A silver nitrate stain for α2 cells in human pancreatic islets. Acta Soc. Med. upsalien. 73, 243–270 (1967)
Like, A. A.: The ultrastructure of the secretory cells of the islets of Langerhans in man. Lab. Invest. 16, 937–951 (1967)
Osaka, M., Sasagawa, T., Fujita, T.: Endocrine cells in human jejunum and ileum: An electron microscope study of biopsy materials. Arch. histol. jap. 35, 235–248 (1973)
Pearse, A. G. E., Polak, J. M.: Bitunctional reagents as vapour and liquid phase fixatives for immunohistochemistry. Abstract Micro '74. Proc. roy. micr. Soc. 9, 67 (1974)
Pearse, A. G. E., Polak, J. M.: Bifunctional reagents as vapour and liquid phase fixatives for immunohistochemistry. Histochem. J. 103 (in press) (1975)
Pearse, A. G. E., Polak, J. M., Bloom, S. R., Adams, C., Dryburgh, J. R., Brown, J. C.: Enterochromaffin cells of the mammalian small intestine as the source of motilin. Virchows Arch. B Cell Pathol. 16, 111–120 (1974)
Polak, J. M., Pearse, A. G. E., Heath, C. M.: Complete identification of endocrine cells in the gastrointestinal tract: Use of the semithin-thin section technique to identify motilin cells in human and animal intestine. Gut 16 (in press) (1975)
Polak, J. M., Bloom, S., Coulling, I., Pearse, A. G. E.: Immunofluorescent localization of enteroglucagon cells in the gastrointestinal tract of the dog. Gut 12, 311–318 (1971)
Polak, J. M., Bloom, S. R., Kuzio, M., Brown, J. C., Pearse, A. G. E.: Cellular localization of gastric inhibitory polypeptide in the duodenum and jejunum. Gut 14, 284–288 (1973)
Polak, J. M., Pearse, A. G. E., Garaud, J-C., Bloom, S. R.: Cellular localization of a vasoactive intestinal peptide in the mammalian and avian gastrointestinal tract. Gut 15, 720–724 (1974)
Sevier, A. C., Munger, B. L.: A silver method for paraffin sections of neural tissue. J. Neuropath. exp. Neurol. 24, 130–135 (1965)
Singh, I.: Argyrophil and argentaffin reactions in individual granules of enterochromaffin cells of reserpine treated guinea-pigs. Z. Zellforsch. 81, 501–510 (1967)
Solcia, E., Capella, C., Vassallo, G., Buffa, R. Endocrine cells of the gastric mucosa. Int. Rev. Cytol. (in press) (1974a)
Solcia, E., Pearse, A. G. E., Grube, D., Kobayashi, S., Bussolati, G., Creutzfeldt, W., Gepts, W.: Revised Wiesbaden Classification of gut endocrine cells. Rendic. Gastroenterol. 5, 13–16 (1973)
Solcia, E., Polak, J. M., Buffa, R., Capella, C., Pearse, A. G. E.: Endocrine cells of the intestinal mucosa. In: International Symposium on Gastrointestinal Hormones (J. C. Thompson, ed.) Austin and London: University of Texas Press (in press) 1974b
Solcia, E., Vassallo, G., Capella, C.: Cytology and cytochemistry of hormone producing cells of the upper gastrointestinal tract. In: Origin chemistry, physiology and pathophysiology of the gastrointestinal hormones, p. 3–29. Stuttgart: Schattauer 1970
Vassallo, G., Capella, C., Solcia, E.: Grimelius' silver stain for endocrine cell granules, as shown by electron microscopy. Stain Technol. 46, 3–13 (1971)
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Buffa, R., Polak, J.M., Pearse, A.G.E. et al. Identification of the intestinal cell storing gastric inhibitory peptide. Histochemistry 43, 249–255 (1975). https://doi.org/10.1007/BF00499706
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DOI: https://doi.org/10.1007/BF00499706