Summary
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1.
Intravenous injection of leukotriene C4 (1 nmol × kg−1) caused substantial plasma exudation in anesthetized guinea pigs, as evidenced by marked hemoconcentration 15% in 5 min) and significant extravasation of Evans blue.
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2.
Fluorometric quantitation of Evans blue content in 38 selected tissues documented that leukotriene C4 caused significant plasma extravasation throughout the body, except for the brain, stomach, duodenum, colon and gonads.
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3.
In particular, the respiratory and the uro-genital tracts, but also the conjunctiva, the esophagus, the bile ducts and the umbilical ligaments, were very sensitive to the edemapromoting effect of leukotriene C4.
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4.
Intravenous injection of leukotriene D4 (1 nmol × kg −1) or E4 (5 nmol × kg−1) evoked plasma extravasation with a distribution and magnitude that was similar to that induced by leukotriene C4.
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5.
It is concluded that the three major constituents of slow reacting substance of anaphylaxis (SRS-A), leukotrienes C4, D4 and E4, cause a generalized and extensive plasma exudation that is consistent with the proposal that these leukotrienes are important mediators of inflammation.
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Hua, XY., Dahlén, SE., Lundberg, J.M. et al. Leukotrienes C4, D4 and E4 cause widespread and extensive plasma extravasation in the guinea pig. Naunyn-Schmiedeberg's Arch. Pharmacol. 330, 136–141 (1985). https://doi.org/10.1007/BF00499906
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DOI: https://doi.org/10.1007/BF00499906