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Substance P as neurogenic mediator of antidromic vasodilation and neurogenic plasma extravasation

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Summary

  1. 1.

    Antidromic vasodilation and neurogenic plasma extravasation were induced by antidromic stimulation of the saphenous nerve in guanethidine-treated rats. Vasodilation was measured by the change of outflow from the femoral vein and plasma extravasation was determined by Evans blue exudation.

  2. 2.

    Antidromic vasodilation was reduced by 85% in adult rats which were pretreated with capsaicin on the second day of life.

  3. 3.

    Antidromic vasodilation was inhibited by 46% after pretreatment with cimetidine plus mepyramine and by 64% after pretreatment with compound 48/80, but was not affected by pretreatment with cimetidine, atropine, methysergide, or indomethacin. Similarly, neurogenic plasma extravasation was reduced by 50% after pretreatment with cimetidine plus mepyramine, and by 88% after pretreatment with compound 48/80, but was not altered after pretreatment with indomethacin.

  4. 4.

    Infusion of substance P into the femoral artery dose-dependently produced vasodilation (threshold: 0.1 pmol min−1) and plasma extravasation (threshold: 0.5 pmol·min−1). Vasodilation induced by substance P was inhibited by 47% after pretreatment with cimetidine plus mepyramine and by 58% after pretreatment with compound 48/80. Plasma extravasation induced by substance P was reduced by 61% after pretreatment with cimetidine plus mepyramine and by 81% after pretreatment with compound 48/80. Pretreatment with indomethacin had no influence on substance P-induced vasodilation and plasma extravasation.

  5. 5.

    It is concluded that vasodilation and plasma extravasation following antidromic stimulation of sensory nerves are initiated by peripheral release of substance P from chemosensitive pain fibres. The actions of substance P include, besides direct effects, release of histamine from mast cells.

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  1. Institut für Experimentelle und Klinische Pharmakologie, Universitätsplatz 4, A-8010, Graz, Austria

    Fred Lembeck & Peter Holzer

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  1. Fred Lembeck
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  2. Peter Holzer
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Lembeck, F., Holzer, P. Substance P as neurogenic mediator of antidromic vasodilation and neurogenic plasma extravasation. Naunyn-Schmiedeberg's Arch. Pharmacol. 310, 175–183 (1979). https://doi.org/10.1007/BF00500282

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  • DOI: https://doi.org/10.1007/BF00500282

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