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Release of histamine by substance P

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Summary

  1. 1.

    The basic peptide substance P causes histamine release from peritoneal mast cells of the rat in vitro whereas the closely related neutral peptides eledoisin and physalaemin do not.

  2. 2.

    Infusion of substance P (7.4 nmol min−1), but not of eledoisin (8.4 nmol min−1) or physalaemin (7.9 nmol min−1), into the rat hindquater preparation caused a more than 4-fold increase of the histamine content in the venous outflow. The outflow of 5-HT remained unchanged under infusion of all three peptides.

  3. 3.

    No histamine depletion in the skin of the rat hind paw was observed following antidromic stimulation of the saphenous nerve or cutaneous application of mustard oil. Infusion of substance P (7.4 nmol min−1) caused a 47% depletion of histamine in the paw skin although only a small proportion of the infused substance P seemed to enter the tissue from the blood vessels.

  4. 4.

    The results further substantiate the view that substance P upon release from peripheral nerve endings induces release of histamine from cutaneous mast cells, a mechanism which contributes to neurogenic vasodilatation and plasma extravasation.

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References

  • Arvier PT, Chahl LA, Ladd RJ (1977) Modification by capsaicin and compound 48/80 of dye leakage induced by irritants in the rat. Br J Pharmacol 59:61–68

    Google Scholar 

  • Benditt EP, Wong RL, Arase M, Roeper E (1955) 5-Hydroxytryptamine in mast cells. Proc Soc Exp Biol (NY) 90:303–304

    Google Scholar 

  • Bhattacharya BK, Lewis GP (1956) The release of 5-hydroxytryptamine by histamine liberators. Br J Pharmacol 11:202–208

    Google Scholar 

  • Bill A, Stjernschantz J, Mandahl A, Brodin E, Nilsson G (1979) Substance P: release on trigeminal nerve stimulation, effects in the eye. Acta Physiol Scand 106:371–373

    Google Scholar 

  • Cuello AC, Polak JM, Pearse AGE (1976) Substance P: a naturally occurring transmitter in human spinal cord. Lancet 13:1054–1056

    Google Scholar 

  • Cuello AC, Del Fiacco M, Paxinos G (1978) The central and peripheral ends of the substance P containing sensory neurones in the rat trigeminal system. Brain Res 152:499–509

    Google Scholar 

  • Douglas WW, Feldberg W, Paton WDM, Schachter M (1951) Distribution of histamine and substance P in the wall of the dogs digestive tract. J Physiol 115:163–176

    Google Scholar 

  • Foreman JC, Jordan CC (1980) Comparison of the activities of some peptides in human skin and as histamine releasing agents. Proc Br Pharmacol Soc (London) C 57: 55

    Google Scholar 

  • Gamse R, Holzer P, Lembeck F (1980) Decrease of substance P in primary afferent neurones and impairment of neurogenic plasma extravasation by capsaicin. Br J Pharmacol 68:207–213

    Google Scholar 

  • Geipert F, Lembeck F (1969) Differentiation of substance P and physalaemin. Naunyn-Schmiedebergs Arch Pharmacol 265:225–232

    Google Scholar 

  • Hägermark Ö, Hökfelt T, Pernow B (1978) Flare and itch induced by substance P in human skin. J Invest Dermatol 71:233–235

    Google Scholar 

  • Hökfelt T, Kellerth JO, Nilsson G, Pernow B (1975) Experimental immunohistochemical studies on the localization and distribution of substance P in cat primary sensory neurones. Brain Res 100:235–252

    Google Scholar 

  • Jasani B, Kreil G, Mackler BF, Stanworth DR (1979) Further studies on the structural requirements for polypeptide-mediated histamine release from rat mast cells. Biochem J 181:623–632

    Google Scholar 

  • Johnson AR, Erdös EG (1973) Release of histamine from mast cells by vasoactive peptides. Proc Soc Exp Biol Med 142:1252–1256

    Google Scholar 

  • Keller R (1958) Zur Bindung von Histamin und Serotonin in den Mast Zellen. Arzneim Forsch 8:390

    Google Scholar 

  • Kiernan JA (1975) A pharmacological and histological investigation of the involvement of mast cells in cutaneous axon reflex vasodilatation. Quart J Exp Physiol 60:123–130

    Google Scholar 

  • Kitada C, Ashida Y, Maki Y, Fujino M, Hirai Y, Yasuhara T, Nakajima T, Takeyama M, Koyama K, Yajima H (1980) Synthesis of granuliberin-R and various fragment peptides and their histamine-releasing activities. Chem Pharm Bull 28:887–892

    Google Scholar 

  • Lembeck F, Donnerer J (1981) Postocclusive cutaneous vasodilatation mediated by substance P. Naunyn-Schmiedeberg's Arch Pharmacol 316:165–171

    Google Scholar 

  • Lembeck F, Geipert F, Starke K (1968) Sialogene Wirkung von Peptiden bei Hühnern. Naunyn-Schmiedebergs Arch Pharmacol 261:422–433

    Google Scholar 

  • Lembeck F, Holzer P (1979) Substance P as neurogenic mediator of antidromic vasodilation and neurogenic plasma extravasation. Naunyn-Schmiedeberg's Arch Pharmacol 310:175–183

    Google Scholar 

  • Lembeck F, Holzer P, Schweditsch M, Gamse R (1978) Elimination of substance P from the circulation of the rat and its inhibition by bacitracin. Naunyn-Schmiedeberg's Arch Pharmacol 305:9–16

    Google Scholar 

  • Mayer N, Lembeck F, Saria A, Gamse R (1979) Substance P: characteristics of binding to synaptic vesicles of rat brain. Naunyn-Schmiedeberg's Arch Pharmacol 306:45–51

    Google Scholar 

  • Morton CR, Chahl LA (1980) Pharmacology of the neurogenic oedema response to electrical stimulation of the saphenous nerve in the rat. Naunyn-Schmiedeberg's Arch Pharmacol 314:271–276

    Google Scholar 

  • Olgart L, Gazelius B, Brodin E, Nilsson G (1977) Release of substance P like immunoreactivity from the dental pulp. Acta Physiol Scand 101:510–512

    Google Scholar 

  • Parratt JR, West GB (1957) Release of 5-hydroxytryptamine and histamine from tissues of the rat. J Physiol (London) 137:169–178

    Google Scholar 

  • Riley JF, West GB (1958) 5-Hydroxytryptamine in mast cells. Ann NY Acad Sci 73:204

    Google Scholar 

  • Rüegg M, Jaques R (1977) Histamine releasing effect of a corticotropin derivate. Pharmacology 15:134–142

    Google Scholar 

  • Samter M, Czarny P (1971) Secondary cells involved in the allergic reaction: eosinophils, basophils, neutrophils. In: Samter M (ed): Immunologic diseases, vol I, 2nd edit. Little Brown, Boston, pp 375–399

    Google Scholar 

  • Shore PA, Burkhalter A, Cohn VH (1959) A method for the fluorometric assay of histamine in tissues. J Pharmacol Exp Ther 127:182–186

    Google Scholar 

  • Skofitsch G, Saria A, Holzer P, Lembeck F (1981) Determination of histamine by high performance liquid chromatography after condensation with o-phthaldialdehyde. J Chromatography (in press)

  • Stach W (1961) Morphologische Beziehungen zwischen Mastzellen und vegetativer Endformation. Z Mikroskop Anatom Forsch 67:257–280

    Google Scholar 

  • Uvnäs B (1965) Der Mechanismus der Histaminfreisetzung aus Mastzellen. Naunyn-Schmiedeberg's Arch Pharmacol 250:137–149

    Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology of the Medical Faculty, University of Ljubjana, Vrazov trg 2, YU-61005, Ljubljana, Yugoslavia

    F. Erjavec & Tatjana Florjanc-Irman

  2. Pain Research Commission of the Austrian Academy of Sciences, Universitätsplatz 4, A-8010, Graz, Austria

    F. Lembeck & G. Skofitsch

  3. Institute of Experimental and Clinical Pharmacology of the University of Graz, Universitätsplatz 4, A-8010, Graz, Austria

    J. Donnerer, A. Saria & P. Holzer

Authors
  1. F. Erjavec
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  2. F. Lembeck
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  3. Tatjana Florjanc-Irman
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  4. G. Skofitsch
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  5. J. Donnerer
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  6. A. Saria
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  7. P. Holzer
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Erjavec, F., Lembeck, F., Florjanc-Irman, T. et al. Release of histamine by substance P. Naunyn-Schmiedeberg's Arch. Pharmacol. 317, 67–70 (1981). https://doi.org/10.1007/BF00506259

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  • DOI: https://doi.org/10.1007/BF00506259

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