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Cloning, expression, and tissue distribution of a fifth melanocortin receptor subtype

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Abstract

The melanocortin (MC) peptides mediate a diverse spectrum of biological activities in both the central nervous system and peripheral tissues by interacting with specific guanine nucleotide binding (G protein)-coupled receptors. Previously, four human melanocortin receptor subtypes have been cloned and characterized. In this study, we have isolated mouse complementary DNA (cDNA) and human genomic clones encoding a fifth melanocortin receptor subtype, MC5. Melanocortin peptide stimulation of human MC5, transiently expressed in COS 1 cells, results in activation of adenylate cyclase with the following rank order of potency: [Nle4, D-Phe7]-α-MSH (melanocyte stimulating hormone) > ACTH (1–24) (adrenocorticotropic hormone) > α-MSH > β-MSH > γ-MSH. Northern blot hybridization, ribonuclease protection, and reverse transcription/polymerase chain reaction assays indicate that mouse MC5 mRNA is most abundant in skeletal muscle and brain. Lower but detectable levels of MC5 mRNA are also found in RT2-2 retinal neuronal cells, lung, testis, spleen, heart, kidney, and liver.

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Authors and Affiliations

  1. Department of Psychobiological Disorders, Dept. 404, Bristol-Myers Squibb Company, 5 Research Parkway, 06492, Wallingford, Connecticut

    Zahra Fathi, Lawrence G. Iben & Eric M. Parker

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  1. Zahra Fathi
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  2. Lawrence G. Iben
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  3. Eric M. Parker
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Fathi, Z., Iben, L.G. & Parker, E.M. Cloning, expression, and tissue distribution of a fifth melanocortin receptor subtype. Neurochem Res 20, 107–113 (1995). https://doi.org/10.1007/BF00995160

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