Abstract
The present study was designed to establish the effects of cytokines on soluble ICAM-1 (sICAM-1) production by human endothelial cells (EC) and ICAM-1 expression on these cells and the effects of purified sICAM-1 on lympho-cyte-EC adhesion. Expression of ICAM-1 and production of sICAM-1 were measured by a specific ELISA method. ICAM-1 expression was enhanced by IL-1β, TNF-α, and most effectively by IFN-γ. IL-4, IL-6, M-CSF, or GM-CSF showed no effects on ICAM-1 expression. IL-4 (100 units/ml) or IL-6(100 units/ml) abolished the enhancing effect of IL-1β, while TNF-α (1, 10, 100 units/ml) synergized with IL-1β to promote ICAM-1 expression in EC. In contrast with the transient increase of cell-associated ICAM-1 expression after activiation by IL-1β, which peaked 40 h poststimulation and declined thereafter, sICAM-1 continued to accumulate in culture supernatants even after 48 h poststimulation in IL-1β-stimulated EC. IL-1β treatment resulted in an increase in adhesion. sICAM-1, purified from cell-free supernatants obtained after a 48-h culture of EC in IL-1β by affinity chromatography using monoclonal ICAM-1 antibody coupled to Sepharose beads, significantly inhibited lymphocyte EC adhesion. Preincubation of lymphocytes with conditioned medium of EC cultured with 100 units/ml IL-1β for 48 h, which contained a considerable amount of sICAM-1, resulted in a significant inhibition of lymphocyte adhesion to IL-1β-stimulated EC. These results suggest that there is a cumulative increase in sICAM-1 concentration in the vicinity of cytokine-stimulated EC and that this sICAM-1 modulates ICAM-1-mediated cell to cell interaction.
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References
Larson, R. S. andT. A. Springer, 1990. Structure and function of leukocyte integrins.Immunol. Rev. 114: 181–217.
Cavender, D., D. Haskard, N. Foster, andM. Ziff. 1987. Superinduction of T lymphocyteendothelial cell (EC) binding by treatment of EC with interleukin 1 and protein synthesis inhibitors.J. Immunol. 138:2149–2154.
Yu, C. L., D. O. Haskard, A. R. Cavender, A. R. Johnson, andM. Ziff. 1985. Human gamma interferon increases the binding of T lymphocytes to endothelial cells.Clin. Exp. Immunol. 62:554–560.
Marlin, S. D., andT. A. Springer. 1987. Purified intercellular adhesion molecule-1 (ICAM-1) is a ligand for lymphocyte function-associated antigen (LFA-1).Cell 51:813–819.
Rothlein, R. M. Czajkowski, M. M. O'Neili, S. D. Marlin, E. Mainolfi, andV. J. Merluzzi. 1988. Induction of intercellular adhesion molecule 1 on primary and continuous cell lines by pro-inflammatory cytokines. Regulation by pharmacologic agents and neutralizing antibodies.J. Immunol. 141:1665–1669.
Dustin, M. L., K. H. Singer, D. T. Tuck, andT. A. Springer. 1988. Adhesion of T lymphocytes to epidermal keratinocytes is regulated by interferon gamma and is mediated by intercellular adhesion molecule-1 (ICAM-1).J. Exp. Med. 167:1323–1340.
Smith, C. W., S. D. Marlin, R. Rothlein, C. Toman, andD. C. Anderson. 1989. Cooperative interaction of LFA-1 and Mac-1 with intercellular adhesion molecule-1 in facilitating adherence and transendothelial migration of human neutrophils in vitro.J. Clin. Invest. 83:2008–2017.
Dustin, M. L., andT. A. Springer. 1988. Lymphocyte function-associated antigen-1 (LFA-1) interaction with intercellular adhesion molecule-1 (ICAM-1) is one of at least three mechanisms for lymphocyte adhesion to cultured endothelial cells.J. Cell Biol. 107:321–331.
Pober, J. S., M. A. Gimbrone, Jr., L. A. Lapierre, D. L. Mendrick, R. Rothlein, andT. A. Springer. 1986. Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immune interferon.J. Immunol. 137:1893–1896.
Rothlein, R., E. A. Mainolfi, M. Czajkowski, andS. D. Marlin. 1991. A form of circulating ICAM-1 in human serum.J. Immunol. 147:3788–3793.
Marlin, S. D., D. E. Staunton, T. A. Springer, C. Stratowa, W. Sommergruber, andV. J. Merluzzi. 1990. A soluble form of intercellular adhesion molecule-1 inhibits rhinovirus infection.Nature 344:70–72.
Becker, J. C., R. Dummer, A. A. Hartmann, G. Burg, andR. E. Schmidt. 1991. Shedding of ICAM-1 from human melanoma cell lines induced by IFN-γ and tumor necrosis factor-α.J. Immunol. 147:4398–4401.
Jaffe, E. A., R. L. Nachman, C. G. Becker, andC. R. Minick. 1973. Culture of human endothelial cells derived from umbilical cord vein.J. Clin. Invest. 52:2745–2756.
Shingu, M., Y. Hashimoto, A. R. Johnson, andE. R. Hurd. 1981. The search for Fc receptors on human tissues and human endothelial cells in culture.Proc. Soc. Exp. Biol. Med. 167:147–155.
Hirano, T., T. Taga, N. Nakano, K. Yasukawa, S. Kashiwamura, K. Shimizu, K. Nakajima, K. H. Pyun, andT. Kishimoto. 1985. Purification to homogeneity and characterization of human B cell differentiation factor (BCDF or BSFp-2).Proc. Natl. Acad. Sci. U.S.A. 82:5490–5494.
Fujii, I., M. Shingu, andM. Nobunaga. 1990. Monocyte activation in early onset rheumatoid arthritis.Ann. Rheum. Dis. 49:497–503.
Shleimer, R. P., S. A. Sterbinsky, J. Kaiser, andC. A. Bickel, D. A. Klunk, K. Tomioka, W. Newman, F. W. Luscinskas, M. A. Gimbrone, Jr., B. W. McIntyre, andB. S. Bochner. 1992. IL-4 induces adhesion of human eosinophils and basophils but not neutrophils to endothelium.J. Immunol. 148:1086–1092.
Abbot, S. E., A. Kaul, C. R. Stevens, andD. R. Blake. 1992. Isolation and culture of synovial microvascular endothelial cells. Characterization and assessment of adhesion molecule expression.Arthritis Rheum 35:401–406.
Oppenheimer-Marks, N., L. S. Davis, D. Tompkins Bogue, J. Ramberg, andP. E. Lipsky. 1991. Differential utilization of ICAM-1 and VCAM-1 during the adhesion and transendothelial migration of human T lymphocytes.J. Immunol. 147:2913–2921.
Hela-Smith, T. H., L. Aneiro, J. H. Korn. 1991. Binding of human rhinovirus and T cells to intercellular adhesion molecule-1 on human fibroblasts.J. Immunol. 147:1831–1836.
Kishimoto, T. K., M. A. Jutila, E. L. Berg, andE. C. Butcher. 1989. Neutrophils Mac-1 and MEL-14 adhesion proteins inversely regulated by chemotactic factors.Science 245:1238–1240.
Haynes, B. F., L. P. Hale, S. M. Denning, P. T. Le, andK. H. Singer. 1989. The role of leukocyte adhesion molecules in cellular interactions: implications for the pathogenesis of inflammatory synovitis.Springer Semin. Immunopathol. 11:163–185.
Cush, J. J., andP. E. Lipsky. 1988. Phenotypic analysis of synovial tissue and peripheral blood lymphocytes isolated from patients with rheumatoid arthritis.Arthritis Rheum. 31:1230–1238.
Van Seventer, G. A., Y. Shimizu, K. J. Horgan, andS. Shaw. 1990. The LFA-1 ligand ICAM-1 provides an important costimulatory signal for T cell receptor-mediated activation of resting T cells.J. Immunol. 144:4579–4586.
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Hashimoto, M., Shingu, M., Ezaki, I. et al. Production of soluble ICAM-1 from human endothelial cells induced by IL-1β and TNF-α. Inflammation 18, 163–173 (1994). https://doi.org/10.1007/BF01534557
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DOI: https://doi.org/10.1007/BF01534557