Abstract
The alteration of renal deposition of mercury (Hg) after mercury vapor (Hgo) exposure was studied in mice pretreated with acivicin, a potent and irreversible inhibitor of γ-glutamyltranspeptidase (GGT). Pretreatment with acivicin decreased renal Hg concentration by about 60% and significantly increased Hg concentration in the urine compared with the non-treated group. The results suggest that renal deposition of Hg after Hgo exposure depends on renal GGT, which plays an important role in the uptake of GSH or GSH conjugates filtered through the glomeruli. It seems that the mechanism of renal Hg deposition after Hgo exposure is similar to that after exposure to ionic Hg: a GGT-mediated incorporation of an Hg-GSH complex into renal tubular cells. The acivicin pretreatment after Hgo exposure did not affect Hg concentrations in the liver and erythrocytes.
References
Akagi H, Nishimura H (1991) Speciation of mercury in the environment. In: Suzuki T, Imura N, Clarkson TW (eds), Advances in mercury toxicology. Plenum Press, New York, pp 53–76
Berlin M (1990) Mercury. In: Friberg L, Nordberg GF, Vouk VB (eds) Handbook on the toxicology of metals. Elsevier, Amsterdam, pp 387–445
Berlin M, Nordberg G, Serenius F (1969) On the site and mechanism of mercury vapor resorption in the lung. Arch Environ Health 18: 42–50
Clarkson TW, Hursh JB, Sager PR, Syversen TLM (1988) Mercury. In: Clarkson TW, Friberg L, Nordberg GF, Sager PR (eds) Biological monitoring of toxic metals. Plenum Press, New York, pp 199–246
de Ceaurriz J, Payan JP, Brondeau MT (1994) Role of extracellular glutathione and γ-glutamyltranspeptidase in the disposition and kidney toxicity of inorganic mercury in rats. J Appl Toxicol 14: 201–206
Himeno S, Takekawa A, Imura N (1993) Species difference in hydroperoxide-scavenging enzymes with special reference to glutathione peroxidase in guinea-pigs. Comp Biochem Physiol 104: 27–31
Khayat A, Dencker L (1983) Whole body and liver distribution of inhaled mercury vapor in the mouse: influence of ethanol and aminotriazole pretreatment. J Appl Toxicol 3: 66–74
Kim C-Y, Watanabe C, Satoh H (1995) Effects of buthionine sulfoximine (BSO) on mercury distribution after Hgo exposure. Toxicology 98: 67–72
Lowry OH, Rosebrough, NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193: 265–275
Magos L (1967) Mercury blood interaction and mercury uptake by brain. Environ Res 1: 323–327
Meister A, Tate SS, Griffith OW (1981) gamma-Glutamyltranspeptidase. Methods Enzymol 77: 237–253
Sugata Y, Clarkson TW, Magos L (1976) A radioactive mercury vapor generating and exposing system for small scale animal experiments. Am Ind Hyg J 27: 449–452
Tanaka T, Naganuma A, Imura N (1990) Role of gamma-glutamyl-transpeptidase in renal uptake and toxicity of inorganic mercury in mice. Toxicology 60: 187–198
World Health Organization (1991) Environmental health criteria 118. Inorganic mercury. Geneva, Switzerland, pp 1–115
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Kim, C.Y., Watanabe, C., Kasanuma, Y. et al. Inhibition of γ-glutamyltranspeptidase decreases renal deposition of mercury after mercury vapor exposure. Arch Toxicol 69, 722–724 (1995). https://doi.org/10.1007/s002040050239
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DOI: https://doi.org/10.1007/s002040050239