Abstract.
Evidence against the Bm1P1 protein as a positive transcription factor for barbiturate-mediated induction of cytochrome P450BM-1 in Bacillus megaterium has been provided in our previous report. In the present study, we show that the basal-level expression of the P450BM-1 promoter-xylE transcriptional fusion is significantly reduced when the bm1P1 gene is present on the same plasmid. Moreover, disruption of the chromosomal bm1P1 gene results in enhanced expression of the P450BM-1 promoter-xylE fusion on a multicopy plasmid. By using a binary vector system, we found that expression of the P450BM-1 promoter-xylE fusion from a high-copy plasmid is substantially repressed by expression of bm1P1 from a low-copy plasmid. Taken together, these results suggest that the basal-level expression of P450BM-1 is negatively affected by bm1P1. Possible mechanisms for this control are discussed.
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Received: 2 July 1999 / Accepted: 2 August 1999
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Shaw, GC., Hsueh, YH. & Kao, HS. The Basal-Level Expression of the Cytochrome P450BM-1 Gene Is Negatively Affected by the bm1P1 Gene of Bacillus megaterium . Curr Microbiol 40, 47–50 (2000). https://doi.org/10.1007/s002849910009
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DOI: https://doi.org/10.1007/s002849910009