Abstract
Purpose
To compare the interaction of human organic anion transporter hOAT4 with PDZ proteins between kidney cells and placental cells.
Materials and Methods
PDZ proteins PDZK1 and NHERF1 were transfected into kidney LLC-PK1 cells and placental BeWo cells expressing hOAT4 or hOAT4-Δ, which lacks the PDZ consensus binding site. The interaction of PDZK1 and NHERF1 with hOAT4 and hOAT4-Δ was investigated by measurement of [3H] estrone sulfate uptake, cell surface and total cell expression of hOAT4.
Results
PDZK1 and NHERF1 enhanced hOAT4 activity in LLC-PK1 cells by increasing the cell surface expression of the transporter. In contrasts, these two PDZ proteins had no effect on hOAT4 activity in BeWo cells.
Conclusion
The interaction of PDZ proteins with hOAT4 may be cell-specific. In placenta, a different set of interacting proteins from PDZK1 and NHERF1 may be required to modulate hOAT4 activity.
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Acknowledgment
This work was supported by Grant R01-DK 60034 from the National Institutes of Health (to Dr. Guofeng You).
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Zhou, F., Xu, W., Tanaka, K. et al. Comparison of the Interaction of Human Organic Anion Transporter hOAT4 with PDZ Proteins between Kidney Cells and Placental Cells. Pharm Res 25, 475–480 (2008). https://doi.org/10.1007/s11095-007-9359-4
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DOI: https://doi.org/10.1007/s11095-007-9359-4