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Down-regulation Synaptic Vesicle Protein 2A in the Anterior Temporal Neocortex of Patients with Intractable Epilepsy

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Abstract

Synaptic vesicle protein 2A (SV2A) involvement has been reported in the animal models of epilepsy. The aim of this study was to investigate the expression of SV2A in human intractable epilepsy (IE) brain tissue. Using immunohistochemistry, immunofluorescence, and Western blot, we detected SV2A expression in tissue samples from the anterior temporal neocortex of 33 patients who had been surgically treated for IE. We compared these tissues with nine histologically normal anterior temporal lobe samples from controls. SV2A immunoreactive staining was 0.1651 ± 0.0564 in patient group and 0.2347 ± 0.0187 in the control group (p < 0.05) using immunohistochemistry, and this finding was consistently observed with Western blot analysis (0.1727 ± 0.0471 versus 0.3976 ± 0.0983, p < 0.05). Immunofluorescence staining showed that SV2A was mainly accumulated in neurons. Our findings demonstrate that down-regulation of SV2A is present in patients with temporal lobe epilepsy.

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References

  • Bajjalieh SM, Frantz GD, Weimann JM, McConnell SK, Scheller RH (1994) Differential expression of synaptic vesicle protein 2 (SV2) isoforms. J Neurosci 14:5223–5235

    PubMed  CAS  Google Scholar 

  • Bennett B, Matagne A, Michel P et al (2007) Seletracetam (UCB 44212). Neurotherapeutics 4:117–122

    Article  PubMed  CAS  Google Scholar 

  • Buckley K, Kelly RB (1985) Identification of a transmembrane glycoprotein specific for secretory vesicles of neural and endocrine cells. J Cell Biol 100:1284–1294

    Article  PubMed  CAS  Google Scholar 

  • Chang WP, Sudhof TC (2009) SV2 renders primed synaptic vesicles competent for Ca2+-induced exocytosis. J Neurosci 29(4):883–897

    Article  PubMed  CAS  Google Scholar 

  • Crowder KM, Gunther JM, Jones TA et al (1999) Abnormal neurotransmission in mice lacking synaptic vesicle protein 2A (SV2A). Proc Natl Acad Sci 96:15268–15273

    Article  PubMed  CAS  Google Scholar 

  • Gillard M, Chatelain P, Fuks B (2006) Binding characteristics of levetiracetam to synaptic vesicle protein 2A (SV2A) in human brain and in CHO cells expressing the human recombinant protein. Eur J Pharmacol 536:102–108

    Article  PubMed  CAS  Google Scholar 

  • Janz R, Sudhof TC (1999) SV2C is a synaptic vesicle protein with an unusually restricted localization: anatomy of a synaptic vesicle protein family. Neuroscience 94:1279–1290

    Article  PubMed  CAS  Google Scholar 

  • Janz R, Goda Y, Geppert M, Missler M, Sudhof TC (1999) SV2A and SV2B function as redundant Ca2+ regulators in neurotransmitter release. Neuron 24:1003–1016

    Article  PubMed  CAS  Google Scholar 

  • Kwan P, Brodie MJ (2000) Early identification of refractory epilepsy. N Engl J Med 342:314–319

    Article  PubMed  CAS  Google Scholar 

  • Lynch BA, Lambeng N, Nocka K et al (2004) The synaptic vesicle protein SV2A is the binding site for the antiepileptic drug levetiracetam. Proc Natl Acad Sci 101:9861–9866

    Article  PubMed  CAS  Google Scholar 

  • Regesta G, Tanganelli P (1999) Clinical aspects and biological bases of drug-resistant epilepsies. Epilepsy Res 34:109–122

    Article  PubMed  CAS  Google Scholar 

  • Rogawski MA, Loscher W (2004) The neurobiology of antiepileptic drugs. Nat Rev Neurosci 5:553–564

    Article  PubMed  CAS  Google Scholar 

  • Scheuer ML, Pedley TA (1990) The evaluation and treatment of seizures. N Engl J Med 323:1468–1474

    Article  PubMed  CAS  Google Scholar 

  • Schmidt D, Loscher W (2005) Drug resistance in epilepsy: putative neurobiologic and clinical mechanisms. Epilepsia 46:858–877

    Article  PubMed  CAS  Google Scholar 

  • Sisodiya SM (2003) Mechanisms of antiepileptic drug resistance. Curr Opin Neurol 16:197–201

    Article  PubMed  CAS  Google Scholar 

  • Toering ST, Boer K, de Groot M et al (2009) Expression patterns of synaptic vesicle protein 2A in focal cortical dysplasia and TSC-cortical tubers. Epilepsia 50(6):1409–1418

    Article  PubMed  CAS  Google Scholar 

  • van Vliet EA, Aronica E, Redeker S, Boer K, Gorter JA (2009) Decreased expression of synaptic vesicle protein 2A, the binding site for levetiracetam, during epileptogenesis and chronic epilepsy. Epilepsia 50(3):422–33

    Article  PubMed  CAS  Google Scholar 

  • von Rosenstiel P (2007) Brivaracetam (UCB 34714). Neurotherapeutics 4:84–87

    Article  Google Scholar 

  • Xiao Z, Gong Y, Wang X-F et al (2009) Altered expression of synaptotagmin I in temporal lobe tissue of patients with refractory epilepsy. J Mol Neurosci 38(2):193–200

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors sincerely thank Tiantan Hospital and Xuanwu Hospital of the Capital University of Medical Sciences, Xinqiao Hospital of the Third Military Medical University for support of brain tissue procurement, the patients and their families for their participation in this study, and the local ethics committee and the National Board of the Medical Affairs for their support.

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Authors and Affiliations

  1. Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China

    Guibo Feng, Fei Xiao, Jie Yuan, Zheng Xiao, Zhiqin Xi & Xuefeng Wang

  2. Department of Geriatrics, The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China

    Yang Lu

  3. Medical Department, Chongqing Medical and Pharmaceutical College, Chongqing, China

    Zuchun Huang

Authors
  1. Guibo Feng
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  2. Fei Xiao
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  3. Yang Lu
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  4. Zuchun Huang
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  5. Jie Yuan
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  6. Zheng Xiao
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  7. Zhiqin Xi
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  8. Xuefeng Wang
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Corresponding author

Correspondence to Xuefeng Wang.

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Xuefeng Wang and Zuchun Huang contributed equally to this work.

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Feng, G., Xiao, F., Lu, Y. et al. Down-regulation Synaptic Vesicle Protein 2A in the Anterior Temporal Neocortex of Patients with Intractable Epilepsy. J Mol Neurosci 39, 354–359 (2009). https://doi.org/10.1007/s12031-009-9288-2

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  • DOI: https://doi.org/10.1007/s12031-009-9288-2

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