Abstract
Pacing postconditioning (PPC) induces cardioprotection. The aim of this study was to determine the optimal pacing algorithm and possible influence of gender and diabetes on PPC. Unprotected regional ischemia for 30 min served as negative control and classical PPC (ten cycles of 30 s left ventricular pacing alternated with 30 s right atrial pacing) as positive control. Area at risk and infarct size were determined by blue dye and triphenyltetrazolium chloride staining. For achieving protection, the minimal number of PPC cycles was seven and the minimal duration of a PPC protocol was 200 s. The protective effect of PPC was comparable in male and female hearts, but no protection could be induced by PPC in diabetic hearts. PPC can provide myocardial protection when using at least seven cycles of ventricular pacing. PPC protection is independent of gender, but sensitive to experimental diabetes.
Similar content being viewed by others
References
Hearse, D. J., & Bolli, R. (1992). Reperfusion induced injury: manifestations, mechanisms, and clinical relevance. Cardiovascular Research, 26, 101–108.
Murry, C. E., Jennings, R. B., & Reimer, K. A. (1986). Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium. Circulation, 74, 1124–1136.
Vinten-Johansen, J., Yellon, D. M., & Opie, L. H. (2005). Postconditioning: a simple, clinically applicable procedure to improve revascularization in acute myocardial infarction. Circulation, 112, 2085–2088.
Zhao, Z. Q., Corvera, J. S., Halkos, M. E., Kerendi, F., Wang, N. P., Guyton, R. A., & Vinten-Johansen, J. (2003). Inhibition of myocardial injury by ischemic postconditioning during reperfusion: comparison with ischemic preconditioning. American Journal of Physiology. Heart and Circulatory Physiology, 285, H579–H588.
Staat, P., Rioufol, G., Piot, C., Cottin, Y., Cung, T. T., L'Huillier, I., Aupetit, J. F., Bonnefoy, E., Finet, G., Andre-Fouet, X., & Ovize, M. (2005). Postconditioning the human heart. Circulation, 112, 2143–2148.
Weihrauch, D., Krolikowski, J. G., Bienengraeber, M., Kersten, J. R., Warltier, D. C., & Pagel, P. S. (2005). Morphine enhances isoflurane-induced postconditioning against myocardial infarction: the role of phosphatidylinositol-3-kinase and opioid receptors in rabbits. Anesthesia and Analgesia, 101, 942–949. table of contents.
Vanagt, W. Y., Cornelussen, R. N., Baynham, T. C., Van Hunnik, A., Poulina, Q. P., Babiker, F., Spinelli, J., Delhaas, T., & Prinzen, F. W. (2007). Pacing-induced dyssynchrony during early reperfusion reduces infarct size. Journal of the American College of Cardiology, 49, 1813–1819.
Ferdinandy, P., Schulz, R., & Baxter, G. F. (2007). Interaction of cardiovascular risk factors with myocardial ischemia/reperfusion injury, preconditioning, and postconditioning. Pharmacological Reviews, 59, 418–458.
Vinten-Johansen, J. (2007). Postconditioning: a mechanical maneuver that triggers biological and molecular cardioprotective responses to reperfusion. Heart Failure Reviews, 12, 235–244.
Tissier, R., Waintraub, X., Couvreur, N., Gervais, M., Bruneval, P., Mandet, C., Zini, R., Enriquez, B., Berdeaux, A., & Ghaleh, B. (2007). Pharmacological postconditioning with the phytoestrogen genistein. Journal of Molecular and Cellular Cardiology, 42, 79–87.
Penna, C., Tullio, F., Merlino, A., Moro, F., Raimondo, S., Rastaldo, R., Perrelli, M. G., Mancardi, D., & Pagliaro, P. (2009). Postconditioning cardioprotection against infarct size and post-ischemic systolic dysfunction is influenced by gender. Basic Research in Cardiology, 104, 390–402.
Kannel, W. B., & McGee, D. L. (1979). Diabetes and cardiovascular risk factors: the Framingham study. Circulation, 59, 8–13.
Herlitz, J., Wognsen, G. B., Emanuelsson, H., Haglid, M., Karlson, B. W., Karlsson, T., Albertsson, P., & Westberg, S. (1996). Mortality and morbidity in diabetic and nondiabetic patients during a 2-year period after coronary artery bypass grafting. Diabetes Care, 19, 698–703.
Tosaki, A., Engelman, D. T., Engelman, R. M., & Das, D. K. (1996). The evolution of diabetic response to ischemia/reperfusion and preconditioning in isolated working rat hearts. Cardiovascular Research, 31, 526–536.
Takahashi, M., Ueda, S., Misaki, H., Sugiyama, N., Matsumoto, K., Matsuo, N., & Murao, S. (1994). Carnitine determination by an enzymatic cycling method with carnitine dehydrogenase. Clinical Chemistry, 40, 817–821.
Gamble, J., & Lopaschuk, G. D. (1994). Glycolysis and glucose oxidation during reperfusion of ischemic hearts from diabetic rats. Biochimica et Biophysica Acta, 1225, 191–199.
Ravingerova, T., Stetka, R., Pancza, D., Ulicna, O., Ziegelhoffer, A., & Styk, J. (2000). Susceptibility to ischemia-induced arrhythmias and the effect of preconditioning in the diabetic rat heart. Physiological Research, 49, 607–616.
del Valle, H. F., Lascano, E. C., Negroni, J. A., & Crottogini, A. J. (2003). Absence of ischemic preconditioning protection in diabetic sheep hearts: role of sarcolemmal KATP channel dysfunction. Molecular and Cellular Biochemistry, 249, 21–30.
Babiker, F. A., Lorenzen-Schmidt, I., Mokelke, E., Vanagt, W. Y., Delhaas, T., Waltenberger, J., Cleutjens, J. P., & Prinzen, F. W. (2010). Long-term protection and mechanism of pacing-induced postconditioning in the heart. Basic Research in Cardiology, 105, 523–533.
Blombery, P. A., & Korner, P. I. (1982). Role of aortic and carotid sinus baroreceptors on Valsalva-like vasoconstrictor and heart rate reflexes in the conscious rabbit. Journal of the Autonomic Nervous System, 5, 303–315.
Kin, H., Zhao, Z. Q., Sun, H. Y., Wang, N. P., Corvera, J. S., Halkos, M. E., Kerendi, F., Guyton, R. A., & Vinten-Johansen, J. (2004). Postconditioning attenuates myocardial ischemia-reperfusion injury by inhibiting events in the early minutes of reperfusion. Cardiovascular Research, 62, 74–85.
Gysembergh, A., Margonari, H., Loufoua, J., Ovize, A., Andre-Fouet, X., Minaire, Y., & Ovize, M. (1998). Stretch-induced protection shares a common mechanism with ischemic preconditioning in rabbit heart. American Journal of Physiology, 274, H955–H964.
Shinmura, K., Nagai, M., Tamaki, K., & Bolli, R. (2004). Gender and aging do not impair opioid-induced late preconditioning in rats. Basic Research in Cardiology, 99, 46–55. doi:10.1007/s00395-003-0436-5.
Shinmura, K., Nagai, M., Tamaki, K., & Bolli, R. (2008). Loss of ischaemic preconditioning in ovariectomized rat hearts: possible involvement of impaired protein kinase C epsilon phosphorylation. Cardiovascular Research, 79, 387–394.
Hisamoto, K., Ohmichi, M., Kurachi, H., Hayakawa, J., Kanda, Y., Nishio, Y., Adachi, K., Tasaka, K., Miyoshi, E., Fujiwara, N., Taniguchi, N., & Murata, Y. (2001). Estrogen induces the Akt-dependent activation of endothelial nitric-oxide synthase in vascular endothelial cells. Journal of Biological Chemistry, 276, 3459–3467.
Garratt, K. N., Brady, P. A., Hassinger, N. L., Grill, D. E., Terzic, A., & Holmes, D. R., Jr. (1999). Sulfonylurea drugs increase early mortality in patients with diabetes mellitus after direct angioplasty for acute myocardial infarction. Journal of the American College of Cardiology, 33, 119–124.
Smith, J. W., Marcus, F. I., & Serokman, R. (1984). Prognosis of patients with diabetes mellitus after acute myocardial infarction. The American Journal of Cardiology, 54, 718–721.
Will, J. C., & Casper, M. (1996). The contribution of diabetes to early deaths from ischemic heart disease: US gender and racial comparisons. American Journal of Public Health, 86, 576–579.
Drenger, B., Ostrovsky, I. A., Barak, M., Nechemia-Arbely, Y., Ziv, E., & Axelrod, J. H. (2011). Diabetes blockade of sevoflurane postconditioning is not restored by insulin in the rat heart: phosphorylated signal transducer and activator of transcription 3- and phosphatidylinositol 3-kinase-mediated inhibition. Anesthesiology, 114, 1364–1372.
Zhu, S. G., Xi, L., & Kukreja, R. C. (2011). Type 2 diabetic Obese db/db mice are refractory to myocardial ischemic postconditioning in vivo: potential role for Hsp20, F(1) -ATPase delta, and Echs1. Journal of Cellular and Molecular Medicine. doi:10.1111/j.1582-4934.2011.01376.x.
Przyklenk, K. (2011). Efficacy of cardioprotective 'conditioning' strategies in aging and diabetic cohorts: the co-morbidity conundrum. Drugs & Aging, 28, 331–343.
Ye, Y., Perez-Polo, J. R., Aguilar, D., & Birnbaum, Y. (2011). The potential effects of anti-diabetic medications on myocardial ischemia–reperfusion injury. Basic Research in Cardiology, 106, 925–952.
Acknowledgment
Ward Vanagt gratefully acknowledges the financial support of the Dutch Heart Foundation, grant number 2010T042.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Babiker, F.A., van Golde, J., Vanagt, W.Y. et al. Pacing Postconditioning: Impact of Pacing Algorithm, Gender, and Diabetes on Its Myocardial Protective Effects. J. of Cardiovasc. Trans. Res. 5, 727–734 (2012). https://doi.org/10.1007/s12265-012-9390-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12265-012-9390-7