A mitochondrial defect in brown adipose tissue of the obese (obob) mouse: Reduced binding of purine nucleotides and a failure to respond to cold by an increase in binding

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Abstract

Atractyloside-insensitive binding of purine nucleotides is reduced in brown adipose tissue mitochondria of the obese (obob) mouse. Exposure of the obob mouse to 4°C does not induce the usual increase in binding. Atractyloside-insensitive binding of purine nucleotides is believed to be a measure of the heat-producing proton conductance pathway in brown adipose tissue mitochondria. It is, therefore, suggested that the impaired thermogenesis of the obob mouse is due to a defect in this pathway in the mitochondria of the brown adipose tissue, the major thermogenic tissues in rodents. The greater metabolic efficiency which would result from a reduced operation of this pathway might be the basis for the obesity in the obob mouse.

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