3-methylindole inhibits lipid peroxidation

https://doi.org/10.1016/0006-291X(87)91606-8Get rights and content

Abstract

The mechanism of pneumotoxicity of 3-methylindole has been postulated to occur via protein alkylation or lipid peroxidation. This report describes the effects of the addition of 3-methylindole to goat lung microsomes to evaluate the possibility that this xenobiotic may increase NADPH-supported lipid peroxidation. Concentrations of malondialdehyde were measured as an index of lipid peroxidation. Instead of a stimulation of lipid peroxidation by 3-methylindole, a complete inhibition of lipid peroxidation was produced by concentrations of 3-methylindole as low as 10 μM. The addition of 3-methylindole to actively peroxidizing microsomes (NADPH-supported) caused an immediate cessation of malondialdehyde production. These results demonstrate that 3-methylindole pneumotoxicity does not proceed by a mechanism of lipid peroxidation, but in fact, this molecule may act as an effective antioxidant to prevent lipid peroxidation in pulmonary tissue.

References (24)

  • J.C. Huijzer et al.

    Toxicol. Appl. Pharmacol

    (1987)
  • M.R. Nocerini et al.

    Toxicol. Appl. Pharmacol

    (1985)
  • M.R. Nocerini et al.

    Tox. Lett

    (1985)
  • S. Kubow et al.

    J. Biol. Chem

    (1984)
  • S. Kubow et al.

    Biochem. Pharmacol

    (1985)
  • P.R. Miles et al.

    Biochem. Pharmacol

    (1980)
  • C. Mottley et al.

    J. Biol. Chem

    (1986)
  • H.G. Shertzer et al.

    Exper. Molec. Pathol

    (1987)
  • M.T. Yokoyama et al.

    Appl. Microbiol

    (1979)
  • E.S. Fiala et al.

    Annu. Rev. Nutr

    (1985)
  • R.G. Breeze et al.

    Adv. Vet. Sci. Comp. Med

    (1982)
  • E.L. Wynder et al.
  • Cited by (12)

    View all citing articles on Scopus
    View full text