Quantitative studies of norepinephrine uptake by synaptosomes

https://doi.org/10.1016/0006-2952(68)90354-7Get rights and content

Abstract

Nerve-ending particles (synaptosomes) isolated from rat brain were studied as a model system for synaptic function with particular reference to the ionic conditions required for uptake of norepinephrine. For optimal uptake, Na+ and K+ were required at levels of 140 mM and 2–3 mM respectively, which are essentially physiological levels for these cations. The inhibition of norepinephrine uptake by reserpine and by higher concentrations of K+ was linear with the logarithm of the inhibitor concentration. The Vmax and Km for norepinephrine uptake were 0·10 μg/min/g and 0·56 × 10−6 M respectively.

References (18)

  • S.H. Snyder et al.

    Life Sci.

    (1965)
  • E.W. Maynert et al.

    Life Sci.

    (1964)
  • D.F. Bogdanski et al.

    Life Sci.

    (1966)
  • C.O. Hebb et al.

    J. Physiol., Lond.

    (1958)
  • V.P. Whittaker et al.

    Biochem. J.

    (1964)
  • E. De Robertis et al.

    J. Neurochem.

    (1962)
  • R.W. Colburn et al.

    Nature, Lond.

    (1967)
  • A.F. Huxley et al.

    J. Physiol. Lond.

    (1951)
  • R.J. Baldessarini et al.

    Science

    (1966)
There are more references available in the full text version of this article.

Cited by (83)

View all citing articles on Scopus
View full text