The effect of tubular damage by mercuric chloride on kidney function and some urinary enzymes in the dog

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Abstract

Alkaline and acid phosphatases, β-glucosidase, β-galactosidase, N-acetyl-β-glucosaminidase and lactate dehydrogenase were monitored in the urine and serum of dogs with renal tubular damage induced by a series of increasing doses of mercuric chloride. Isocitrate dehydrogenase, glutamate oxaloacetate transaminase and glutamate pyruvate transaminase were assayed in the serum. The functional capacity of the kidney was determined by creatinine, inulin and p-aminohippurate (PAH) clearances and tubular maxima tests. Serum urea levels, total protein in the urine and the specific gravity of the urine were monitored throughout the study. The extent and location of the kidney damage was determined by histological investigation. Evidence is presented that the assay of urinary alkaline and acid phosphatase is the most sensitive method of detecting renal tubular damage in the dog. The clearance of [14C]-propranolol was compared before and after the administration of mercuric chloride. In the presence of tubular damage the blood half-life of propranolol and the rate of excretion of metabolites in the urine were increased.

References (35)

  • C. Breedis et al.

    Alkaline phosphatase level in urine in relation to renal injury

    Arch. Pathol.

    (1943)
  • M.R. Schoenfeld

    Acid phosphatase activity in ureteral urine

    J. Am. Med. Ass.

    (1965)
  • J.P. Haslet et al.

    Urinary muramidase and renal disease

    New Engl. J. Med.

    (1968)
  • D. Robinson et al.

    Rat-urine glycosidases and kidney damage

    Biochem. J.

    (1967)
  • R.G. Price et al.

    A comparison of the β-glycosidase excretion during kidney damage induced by 4-nitrophenylarsonic acid and by rabbit anti-rat kidney antibodies

    European J. Clin. Invest.

    (1970)
  • W.P. Raab

    Diagnostic value of urinary enzyme determinations

    Clin. Chem.

    (1972)
  • C.E. McCreight et al.

    Sequence of morphological and functional changes in renal epithelial following heavy metal poisoning

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