Elsevier

Life Sciences

Volume 29, Issue 7, 17 August 1981, Pages 663-671
Life Sciences

Characterization and regional distribution of calcitonin binding sites in the rat brain

https://doi.org/10.1016/0024-3205(81)90018-7Get rights and content

Abstract

Binding sites for calcitonin (CT), as assayed by the displacable binding of [125-I] iodo salmon CT ([125-I]sCT), were found on a membrane fraction prepared from rat brain. The half times of association varied between 23 and 7 min as a function of the temperatures used in the incubation medium, ranging from 6° to 37°C. Salmon CT in amounts as low as 10−10 M inhibited the binding of [125-I]sCT to the membranes, whereas the virtually biologically inactive free acid of human CT and human CT sulfone did not affect the binding. The specific binding of [125-I]sCT to the membranes was directed to structural and/or conformational features in the COOH-terminal half of salmon CT. 133 to 8,900 times higher amounts of porcine CT and human CT and analogues thereof were required to achieve an inhibition of binding equal to that produced by salmon CT. Sixty-seven percent of specific binding of labeled hormone was not dissociable, even after 6 h of incubation with an excess of unlabeled hormone. [125-I]sCT extracted from the membranes was not degraded, as judged by gel permeation chromatography, and retained binding activity. Specific binding was highest in the hypothalamus, followed by the brainstem. It was intermediate in the midbrain-thalamus and the striatum, lower in the cortex and negligible in the hippocampus, and cerebellum and the spinal cord.

References (39)

  • S.J. Marx et al.

    J. Biol. Chem.

    (1973)
  • S.F. Queener et al.

    J. Biol. Chem.

    (1975)
  • A.H. Tashjian et al.

    Rec. Progr. Horm. Res.

    (1978)
  • S.J. Marx et al.

    J. Biol. Chem.

    (1974)
  • M. Koida et al.

    Japan. J. Pharmacol.

    (1980)
  • Y. Iwasaki et al.

    Life Sci.

    (1979)
  • M. Yamamoto et al.

    Europ. J. Pharmacol.

    (1979)
  • K.L. Becker et al.

    Brain Res.

    (1980)
  • L.J. Deftos et al.

    Life Sci.

    (1978)
  • A.G.E. Pearse et al.
  • P.L. Munson
  • R. Ardaillou

    Nephron

    (1975)
  • S.J. Marx et al.

    Science

    (1972)
  • J. Sraer et al.

    Endocrinology

    (1974)
  • N. Loreau et al.

    J. Endocr.

    (1978)
  • D. Goltzman

    Endocrinology

    (1980)
  • J.E. Mahaffey et al.
  • J. Moran et al.
  • N.H. Hunt et al.

    Br. J. Cancer

    (1977)
  • Cited by (88)

    View all citing articles on Scopus
    View full text