Codominant and recessive 9-β-D-arabinofuranosyladenine-resistant mutation of baby hamster cells

https://doi.org/10.1016/0027-5107(85)90019-3Get rights and content

Abstract

9-β-D-Arabinosyladenine (araA)-resistant mutants of baby hamster kidney (BHK) cells can be classified into 3 classes. In order to gain a better understanding of the mechanism(s) of resistance and the biochemical basis of cytotoxicity of various purine nucleosides, cell hybrids of the mutant and wild-type cells were made and analyzed. The class I araA-resistant, adenosine-kinase-deficient (AK) allele was shown to be recessive to the wild-type araA-sensitive (AK+) gene. The class II mutant allele, which encodes an altered ribonucleoside diphosphate reductase, was shown to be codominant. The class III mutants show multiple phenotypes, araAr/dAdor/adenosine sensitive (Ados) and alteration in AK activity. The araA-and dAdo-resistant alleles of araS10d, ara-16c, and ara-19a in class III mutant/wild-type hybrid cells are all recessive to the wild-type allele, consistent with a common mechanism of resistance. In contrast the Ados allele of ara-S10d is dominant while those of ara-16c and ara-19a are recessive. The difference may be a reflection of two distinct mechanisms of enhanced Ado sensitivity or, alternatively, it suggests that the sensitivity of the hybrids to Ado is highly dependent on the level of AK activity.

References (16)

  • D. Ayusawa et al.

    Increase in dATP pool in aphidicolin-resistant mutants of mouse FM3A cells

    Biochem. Biophys. Res. Commun.

    (1979)
  • B. Ullman et al.

    Deoxyadenosine metabolism and cytotoxicity in cultured mouse T lymphoma cells: A model for immunodeficiency disease

    Cell

    (1978)
  • S.M. Archer

    Mechanisms of Adenosine Toxicity in Baby Hamster Kidney Cells

  • D. Ayusawa et al.

    Alteration of ribonucleotide reductase in aphidicolin-mutants of mouse FM3A cells with associated resistance to arabinosyladenine and arabinosylcytosine

    Somat. Cell Genet.

    (1981)
  • V.L. Chan et al.

    Isolation and preliminary characterization of 9-β-D-arabinosyladeine-resistant mutants of baby hamster cells

    Somat. Cell Genet.

    (1981)
  • V.L. Chan et al.

    Mutator genes of baby hamster kidney cell

    Mol. Cell Biol.

    (1981)
  • V.L. Chan et al.

    A novel pleiotropic mutation in baby hamster kidney cells causing increased resistance to arabinosyladenine, sensitivity to adenosine and adenosine kinase deficiency

  • R.L. Davidson et al.

    Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol

    Somat. Cell Genet.

    (1976)
There are more references available in the full text version of this article.

Cited by (1)

View full text