Elsevier

Methods in Enzymology

Volume 159, 1988, Pages 118-139
Methods in Enzymology

[11] Use of synergistic pairs of site-selective cAMP analogs in intact cells

https://doi.org/10.1016/0076-6879(88)59013-4Get rights and content

Publisher Summary

The method of using pairs of cAMP analogs to identify cA PK-mediated responses in intact tissues appears to be generally applicable. A number of different cA PK-mediated responses have been demonstrated in several different cell preparations from several different species. These include lipolysis and fatty acid transport in isolated rat adipocytes; phosphorylase activation in rat adipocytes, rat hepatocytes, and bovine neutrophils; phosphoenolpyruvate carboxykinase (PEPCK) gene transcription and mRNAPEPCK induction in continuous cultures of H4 hepatoma cells; and progesterone synthesis and luteinizing hormone (LH) receptor induction in primary cultures of porcine granulose cells. When type I-directed or type II-directed analog pairs are used in vitro, only the appropriate, single isozyme is synergistically activated. This is true for the analog pairs with asterisks when testing a number of isozymes. These include the type I isozyme from rat hepatocytes, bovine neutrophils, rabbit and rat skeletal muscle, and dog thyroid; and the type II isozyme from rat adipocytes, rat hepatocytes, bovine neutrophils, bovine heart, rat skeletal muscle, H4 hepatoma cells, rat liver membranes, and dog thyroid.

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