Autoradiographic localization of leukotriene C4 and D4 binding sites in guinea-pig lung

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Abstract

Binding of [3H] leukotriene C4 and D4 to guinea-pig lung sections was charaterised and binding sites were localized by autoradiography. Both leukotrienes bound to guinea-pig lung sections and membranes with high affinity and with similar charateristics to binding in a membrane preparation. Autoradiography revealed that the distribution of LTC4 and D4 binding sites was markedly different. Smooth muscle and epithelium of central and peripheral airways were densely labelled with [3H]LTC4; vascular smooth muscle and alveolar walls were also labelled. With [3H]LTD4, however, there was no detectable labelling of airways or vessels but subtantial labelling of alveolar walls. This lends futher support that LTC4 and LTD4 binding sites differ and may not be identical with functional receptors.

References (25)

  • R.D. Krell et al.

    Prostaglandins

    (1981)
  • R.T. Bruns et al.

    Life Sci.

    (1983)
  • G.K. Hogaboom et al.

    Biochem. Biophys. Res. Commun.

    (1983)
  • J.B. Cheng et al.

    Biochem. Biophys. Res. Commun.

    (1984)
  • S. Mong et al.

    Eur. J. Pharmacol.

    (1985)
  • P. Norman et al.

    Eur. J. Pharmacol.

    (1987)
  • E.J. Corey et al.

    Tetrahedron Lett.

    (1982)
  • P. Barnes et al.

    Life Sci.

    (1979)
  • P.J. Munson et al.

    Anal Biochem.

    (1980)
  • F.F. Sun et al.

    J. Biol. Chem.

    (1986)
  • R.A. Lewis et al.

    J. Clin. Invest.

    (1984)
  • P.J. Piper

    Physiol. Rev.

    (1984)
  • Cited by (6)

    View full text